Contribution of Myostatin gene polymorphisms to normal variation in lean mass, fat mass and peak BMD in Chinese male offspring
Contribution of Myostatin gene polymorphisms to normal variation in lean mass, fat mass and peak BMD in Chinese male offspring
复制标题
肌肉生长抑制素基因多态性对中国男性后代瘦肉量、脂肪量和峰值骨密度正常变化的影响
DOI：
10.1038/aps.2012.12
复制
发表时间：
2012-05-01
影响因子：
8.2
通讯作者：
Zhang, Zhen-lin
中科院分区：
文献类型：
--
作者：
Yue, Hua;He, Jin-wei;Zhang, Zhen-lin
目的：肌生长抑制素基因是调控骨骼肌生长的转化生长因子- β (tgf - β)家族的一员。肌生长抑制素基因多态性与中国女性骨密度峰值有关。本研究的目的是探讨肌生长抑制素是否在中国男性峰值骨密度、瘦质量（LM）和脂肪质量（FM）的正常变化中发挥作用。方法：招募400个汉族男性子代核心家庭。采用双能x射线吸收仪（DXA）测量人体测量，包括峰值骨密度、体LM和FM。研究的单核苷酸多态性（snp）是通过测序选择的标签snp。采用TaqMan法对rs2293284和+2278G>A进行基因分型，rs3791783采用pcr -限制性片段长度多态性（RFLP）分析进行基因分型。使用定量传递不平衡测试（QTDT）分析snp与人体测量变化的关系。结果：使用QTDT检测家族内关联，没有发现单个SNP或单倍型与任何骨骼部位的峰值骨密度相关。然而，rs3791783被发现与躯干脂肪量显著相关(P
Aim: Myostatin gene is a member of the transforming growth factor-beta (TGF-beta) family that negatively regulates skeletal muscle growth. Genetic polymorphisms in Myostatin were found to be associated with the peak bone mineral density (BMD) in Chinese women. The purpose of this study was to investigate whether Myostatin played a role in the normal variation in peak BMD, lean mass (LM), and fat mass (FM) of Chinese men.Methods: Four hundred male-offspring nuclear families of Chinese Han ethnic group were recruited. Anthropometric measurements, including the peak BMD, body LM and FM were measured using dual-energy X-ray absorptiometry (DXA). The single nucleotide polymorphisms (SNPs) studied were tag-SNPs selected by sequencing. Both rs2293284 and +2278G>A were genotyped using TaqMan assay, and rs3791783 was genotyped with PCR-restriction fragment length polymorphism (RFLP) analysis. The associations of the SNPs with anthropometric variations were analyzed using the quantitative transmission disequilibrium test (QTDT).Results: Using QTDT to detect within-family associations, neither single SNP nor haplotype was found to be associated with peak BMD at any bone site. However, rs3791783 was found to be significantly associated with fat mass of the trunk (P