Octamer-primed cycle sequencing using dye-terminator chemistry.
Octamer-primed cycle sequencing using dye-terminator chemistry.
复制标题
使用染料终止剂化学进行八聚体引发的循环测序。
DOI：
10.1093/nar/26.11.2824
复制
发表时间：
1998
影响因子：
14.9
通讯作者：
Hardin,SH
中科院分区：
文献类型：
--
作者：
Jones,LB;Hardin,SH
八聚体测序技术（OST）是一种使用单个八聚体寡核苷酸来引发循环测序反应的DNA测序方法。这种测序策略比传统的引物步移策略更快，因为对这种优化的八聚体文库的访问消除了与设计和合成基因特异性引物相关的延迟。在这份报告中，OST已被优化的荧光，染料终止剂循环测序反应，以促进平行处理的样品。OST的成功适应anautomated测序平台和八聚体库的设计和访问是朝着开发一个有效的“闭环”DNA测序系统的关键步骤。
Octamer Sequencing Technology, OST, is a method of DNA sequencing using single octamer oligonucleotides to prime cycle sequencing reactions. This sequencing strategy is faster than a traditional primer-walking strategy, since access to this optimized octamer library eliminates delays associated with designing and synthesizing gene specific primers. In this report, OST has been optimized for fluorescent, dye-terminator cycle sequencing reactions to facilitate parallel processing of samples. The successful adaptation of OST to anautomated sequencing platform and the design of and access to an octamer library are critical steps towards developing an efficient ‘closed-loop’ DNA sequencing system.