Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial
Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial
复制标题
DOI：
10.1186/ar3064
复制
发表时间：
2010-01-01
影响因子：
4.9
通讯作者：
Kjaer, Michael
中科院分区：
文献类型：
--
作者：
Helmark, Ida C.;Mikkelsen, Ulla R.;Kjaer, Michael
简介：微透析方法应用于人类膝关节骨关节炎（OA），以揭示软骨和炎症的生化标志物的变化，intrartically和滑膜，在响应一个单一回合的机械关节loading.Methods：31名女性受试者与OA的膝盖被随机分配到非运动（NEX）或运动（EX）组。在急性抗阻运动（25组，10次重复，1次重复最大值的60%）或无（NEx）后，应用腹股沟韧带正下方的外周神经阻滞，并将两个微透析导管定位在关节内和滑膜周围的两个不同隔室中。微透析导管以缓慢的速度灌注（2 μ l/分钟）与林格氏乙酸盐和放射性标记葡萄糖的溶液混合，允许测定相对恢复率（RR）并计算三小时内炎性和软骨生物标志物的间质浓度。在运动后3小时内，在Ex组的膝关节的两个位置中发现白细胞介素（IL）-10显著增加，而NEx组中IL-10随时间保持稳定。IL-6和IL-8显示随时间显著增加，与导管的组和位置无关。软骨寡聚基质蛋白（COMP）减少intraarterically在运动后的时期在Ex组相比NEx group.Conclusions：运动引起的增加，在关节内和周围滑膜浓度的IL-10在一组人类女性膝关节OA。这表明运动对膝关节OA患者的软骨保护性抗炎细胞因子反应具有积极作用，并可能有助于解释运动对OA的有益作用。
Introduction: The microdialysis method was applied to the human knee joint with osteoarthritis (OA) in order to reveal changes in biochemical markers of cartilage and inflammation, intraarticularly and in the synovium, in response to a single bout of mechanical joint loading.Methods: Thirty-one female subjects with OA of the knee were randomized to non-exercise (NEx) or exercise (Ex) groups. Following acute resistance exercise (25 sets of 10 repetitions at 60% of 1 Repetition Maximum) or none (NEx), peripheral nerve blocks just below the inguinal ligament were applied and two microdialysis catheters were positioned in two different compartments, intraarticularly and peri-synovially. The microdialysis catheters were perfused at a slow rate (2 mu l/minute) with a solution of Ringer-acetate and radioactively labelled glucose allowing for determination of relative recovery (RR) and calculation of interstitial concentrations of inflammatory and cartilage biomarkers over a three-hour period.Results: A significant increase of Interleukin (IL) - 10 was discovered in both positions of the knee in the Ex group over the three hours post exercise, whereas IL-10 remained stationary over time in the NEx group. IL-6 and IL-8 displayed significant increases over time regardless of group and position of the catheter. Cartilage oligomeric matrix protein (COMP) decreased intraarticularly in the post exercise period in the Ex group compared to the NEx group.Conclusions: Exercise caused an increase in both intraarticular and peri-synovial concentrations of IL-10 in a group of human females with knee OA. This suggests a positive effect of exercise on a chondroprotective anti-inflammatory cytokine response in patients with knee OA and might contribute to explaining the beneficial effect that exercise has on OA.Trial registration: NCT01090375.