The Metabotropic Glutamate Receptor Subtype 1 Mediates Experience-Dependent Maintenance of Mature Synaptic Connectivity in the Visual Thalamus
The Metabotropic Glutamate Receptor Subtype 1 Mediates Experience-Dependent Maintenance of Mature Synaptic Connectivity in the Visual Thalamus
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DOI：
10.1016/j.neuron.2016.07.035
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发表时间：
2016-09-07
期刊：
影响因子：
16.2
通讯作者：
Kano, Masanobu
中科院分区：
文献类型：
--
作者：
Narushima, Madoka;Uchigashima, Motokazu;Kano, Masanobu
在出生后发育过程中形成的神经回路必须在此后保持稳定，但其机制在很大程度上仍然未知。在这里，我们报告的代谢型谷氨酸受体亚型1（mGluR 1）是必不可少的，在背外侧膝状体核（dLGN）的成熟突触连接的维护。在mGluR 1敲除（mGluR 1-KO）小鼠中，直到出生后第20天（P20）左右，视网膜神经突触的增强和消除才正常发生。然而，在随后的视觉经验依赖的维护阶段，弱retinogeniculate突触新招募。这些变化类似于野生型（WT）小鼠，经历视觉剥夺或失活的mGluR 1的dLGN从P21。重要的是，视觉剥夺在mGluR 1-KO小鼠中是无效的，并且WT小鼠中视觉剥夺诱导的变化通过dLGN中mGluR 1的药理学激活而被挽救。这些结果表明，mGluR 1是至关重要的视觉经验依赖性的维护成熟的突触连接的dLGN。
Neural circuits formed during postnatal development have to be maintained stably thereafter, but their mechanisms remain largely unknown. Here we report that the metabotropic glutamate receptor subtype 1 (mGluR1) is essential for the maintenance of mature synaptic connectivity in the dorsal lateral geniculate nucleus (dLGN). In mGluR1 knockout (mGluR1-KO) mice, strengthening and elimination at retinogeniculate synapses occurred normally until around postnatal day 20 (P20). However, during the subsequent visual-experience-dependent maintenance phase, weak retinogeniculate synapses were newly recruited. These changes were similar to those of wild-type (WT) mice that underwent visual deprivation or inactivation of mGluR1 in the dLGN from P21. Importantly, visual deprivation was ineffective in mGluR1-KO mice, and the changes induced by visual deprivation in WT mice were rescued by pharmacological activation of mGluR1 in the dLGN. These results demonstrate that mGluR1 is crucial for the visual-experience-dependent maintenance of mature synaptic connectivity in the dLGN.