Characterization of aortic aneurysms in cardiovascular disease patients harboring Porphyromonas gingivalis
Characterization of aortic aneurysms in cardiovascular disease patients harboring Porphyromonas gingivalis
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DOI：
10.1111/j.1601-0825.2010.01759.x
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发表时间：
2011-05-01
期刊：
影响因子：
3.8
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
--
作者：
Nakano, K.;Wada, K.;Ooshima, T.
目的：牙龈卟啉单胞菌（Porphyromonas gingivalis）最近在小鼠模型中通过一种新的胆固醇非依赖性机制引起内膜增生，这表明它是心血管疾病的一种病原体特异性特征。本研究的目的是描述心血管疾病患者携带口腔牙龈假单胞菌的主动脉瘤的临床和组织病理学特征。对象与方法：收集76例日本手术患者的主动脉瘤标本，其中31例患者的牙菌斑标本。从每个标本中提取细菌DNA，采用聚合酶链反应检测牙龈假单胞菌。对动脉瘤标本进行组织病理学分析，包括胚胎肌球蛋白重链异构体（SMemb）和S100钙结合蛋白A9 （S100A9）的免疫组化染色。结果：牙菌斑中牙龈假单胞菌阳性受试者远端主动脉动脉瘤的发生率明显高于阴性受试者。在牙菌斑中牙龈假单胞菌阳性的受试者中，S100A9和SMemb的表达也显著增加。另一方面，两组之间的脂肪细胞积累没有显著差异。结论：口腔牙龈假单胞菌患者的主动脉瘤中S100A9和增殖平滑肌细胞的表达高于非口腔牙龈假单胞菌患者。
Objective:Porphyromonas gingivalis was recently shown to cause intimal hyperplasia in a mouse model by a novel cholesterol-independent mechanism, suggesting to be a pathogen-specific feature of cardiovascular diseases. The aim of this study was to characterize the clinical and histopathological features of aortic aneurysms in cardiovascular disease patients harboring oral P. gingivalis.Subject and methods:Aortic aneurysm specimens were collected from 76 Japanese patients who underwent surgery, of whom dental plaque specimens were also collected from 31 patients. Bacterial DNA was extracted from each specimen to detect P. gingivalis by polymerase chain reaction. Histopathological analyses of the aortic aneurysm specimens, including immunohistochemical staining for embryonic myosin heavy chain isoform (SMemb) and S100 calcium-binding protein A9 (S100A9), were also performed.Results:The number of aneurysms occurring in the distal aorta was significantly higher in subjects positive for P. gingivalis in dental plaque compared with those who were negative. The expressions of S100A9 and SMemb were also significantly greater in the subjects positive for P. gingivalis in dental plaque. On the other hand, there were no significant differences in adipocellular accumulation between the groups.Conclusions:These results suggest that aortic aneurysms in patients harboring oral P. gingivalis have greater expression of S100A9 and proliferative smooth muscle cells, which was different from the present patients without oral P. gingivalis.