Structural insight into OprD substrate specificity
Structural insight into OprD substrate specificity
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DOI：
10.1038/nsmb1304
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发表时间：
2007-11-01
影响因子：
16.8
通讯作者：
van den Berg, Bert
中科院分区：
文献类型：
--
作者：
Biswas, Shyamasri;Mohammad, M. Mohammad;van den Berg, Bert
OprD蛋白在革兰氏阴性菌中形成底物特异性外膜通道大家族。我们报道了来自铜绿假单胞菌的OprD的X射线晶体结构，它显示了一个单体的18链β-桶，其特征是非常窄的孔收缩，一边是带正电荷的基本梯子，另一边是带电负性的口袋。OprD中高度保守残基的位置表明该结构代表了OprD通道的总体结构。
OprD proteins form a large family of substrate-specific outermembrane channels in Gram-negative bacteria. We report here the X-ray crystal structure of OprD from Pseudomonas aeruginosa, which reveals a monomeric 18-stranded beta-barrel characterized by a very narrow pore constriction, with a positively charged basic ladder on one side and an electronegative pocket on the other side. The location of highly conserved residues in OprD suggests that the structure represents the general architecture of OprD channels.