Multiplexed DNA detection using a gold nanorod-based fluorescence resonance energy transfer technique.
Multiplexed DNA detection using a gold nanorod-based fluorescence resonance energy transfer technique.
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DOI：
10.1002/bio.2885
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
Qiang Wu;Yan-Hui He;Jianniao Tian;Juanni Zhang;Kun Hu;Yanchun Zhao;Shulin Zhao
中科院分区：
文献类型：
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作者：
Qiang Wu;Yan-Hui He;Jianniao Tian;Juanni Zhang;Kun Hu;Yanchun Zhao;Shulin Zhao
成功构建了一种基于金纳米棒的荧光共振能量转移多重检测DNA的方法。该方法简单、易于操作、选择性好、无需标记探针分子，可以同时分析一个样品中的多个DNA靶点。在信噪比为3时，18-mer、27-mer和30-mer靶标的检测限为0.72、1.0和0.43 nM。3种目标物的回收率分别为96.57- 98.07%、99.12-100.04%和97.29- 99.93%。结果表明，该方法可用于临床样品或生物样品的分析，也可用于开发新的探针，用于真实的样品中分析物的快速、灵敏和高选择性的多重检测。
A fluorescence resonance energy transfer method for multiplex detection DNA based on gold nanorods had been successfully constructed. This method is simple, easy to operate, good selectivity, no requirement to label the probe molecule and can analyze simultaneously multiple targets of DNA in one sample. The limit of detection for the 18-mer, 27-mer and 30-mer targets is 0.72, 1.0 and 0.43 nM at a signal-to-noise ratio of 3. The recoveries of three targets were 96.57-98.07%, 99.12-100.04% and 97.29-99.93%, respectively. The results show that the method can be used to analyze a clinical sample or a biological sample; it also can be used to develop new probes for rapid, sensitive and highly selective multiplex detection of analytes in real samples.