Dietary Anthocyanin-Rich Bilberry Extract Ameliorates Hyperglycemia and Insulin Sensitivity via Activation of AMP-Activated Protein Kinase in Diabetic Mice
Dietary Anthocyanin-Rich Bilberry Extract Ameliorates Hyperglycemia and Insulin Sensitivity via Activation of AMP-Activated Protein Kinase in Diabetic Mice
复制标题
DOI：
10.3945/jn.109.118216
复制
发表时间：
2010-03-01
影响因子：
4.2
通讯作者：
Tsuda, Takanori
中科院分区：
文献类型：
--
作者：
Takikawa, Masahiro;Inoue, Seiya;Tsuda, Takanori
蓝莓或越橘含有大量的花青素，使它们成为膳食花青素最丰富的来源之一。这些浆果被广泛用作新鲜和干果，果酱或果汁。相当多的注意力集中在越橘水果的健康益处上，而不仅仅是它们的抗氧化剂含量或改善视力的能力。在这项研究中，我们测试了膳食越橘提取物（BBE）对2型糖尿病小鼠高血糖和胰岛素敏感性的影响。我们发现饮食BBE通过激活AMP激活蛋白激酶（AMPK）改善高血糖和胰岛素敏感性。膳食BBE显著降低血糖浓度，增强胰岛素敏感性。AMPK在喂食BBE的糖尿病小鼠的白色脂肪组织（WAT）、骨骼肌和肝脏中被激活。这种激活伴随着WAT和骨骼肌中葡萄糖转运蛋白4的上调以及肝脏中葡萄糖产生和脂质含量的抑制。与此同时，乙酰辅酶A羧化酶被灭活，肝脏中的过氧化物酶体增殖物激活受体α、酰基辅酶A氧化酶和肉毒碱棕榈酰转移酶-1A被上调。这些变化导致2型糖尿病的高血糖和胰岛素敏感性改善。这些发现为越橘果实的使用提供了生化基础，并对通过激活AMPK预防和治疗2型糖尿病具有重要意义。J.营养140：527-533，2010.
Blueberries or bilberries contain large amounts of anthocyanins, making them one of the richest sources of dietary anthocyanin. These berries are widely consumed as fresh and dried fruits, jams, or juices. Considerable attention has been focused on the health benefits of bilberry fruits beyond their antioxidant content or their ability to improve vision. In this study, we tested the effect of dietary bilberry extract (BBE) on hyperglycemia and insulin sensitivity in type 2 diabetic mice. We found that dietary BBE ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase (AMPK). Dietary BBE significantly reduced the blood glucose concentration and enhanced insulin sensitivity. AMPK was activated in white adipose tissue (WAT), skeletal muscle, and the liver of diabetic mice fed BBE. This activation was accompanied by upregulation of glucose transporter 4 in WAT and skeletal muscle and suppression of glucose production and lipid content in the liver. At the same time, acetyl-CoA carboxylase was inactivated and PPAR alpha, acyl-CoA oxidase, and carnitine palmitoyltransferase-1A were upregulated in the liver. These changes resulted in improved hyperglycemia and insulin sensitivity in type 2 diabetes. These findings provide a biochemical basis for the use of bilberry fruits and have important implications for the prevention and treatment of type 2 diabetes via activation of AMPK. J. Nutr. 140: 527-533, 2010.