Optical Nano-Constructs Composed of Genome-Depleted Brome Mosaic Virus Doped with a Near Infrared Chromophore for Potential Biomedical Applications
Optical Nano-Constructs Composed of Genome-Depleted Brome Mosaic Virus Doped with a Near Infrared Chromophore for Potential Biomedical Applications
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DOI：
10.1021/nn1028696
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发表时间：
2011-02-01
期刊：
影响因子：
17.1
通讯作者：
Anvari, Bahman
中科院分区：
文献类型：
--
作者：
Jung, Bongsu;Rao, Ayala L. N.;Anvari, Bahman
我们设计了一种光学纳米结构，由基因组缺失的溴花叶病毒掺杂吲哚菁绿（ICG）组成，这是一种经fda批准的近红外（NIR）发色团。构建体高度单分散，与平均直径24.3 nm的标准差为+/- 3.8 nm。它们在物理上是稳定的，在生理温度（37℃）下表现出高度的光学稳定性。使用人支气管上皮细胞，我们证明了体外细胞内光学成像构建的有效性，培养3小时后细胞存活率大于90%。这些结构可以作为一种潜在的无毒和多功能纳米平台，用于特定部位的深层组织光学成像和疾病治疗。
We have engineered an optical nanoconstruct composed of genome-depleted brome mosaic virus doped with indocyanine green (ICG), an FDA-approved near-infrared (NIR) chromophore. Constructs are highly monodispersed with standard deviation of +/- 3.8 nm from a mean diameter of 24.3 nm. They are physically stable and exhibit a high degree of optical stability at physiological temperature (37 degrees C). Using human bronchial epithelial cells, we demonstrate the effectiveness of the constructs for intracellular optical imaging in vitro, with greater than 90% cell viability after 3 h of incubation. These constructs may serve as a potentially nontoxic and multifunctional nanoplatform for site-specific deep-tissue optical imaging, and therapy of disease.