Variations in the structure of airborne bacterial communities in a downwind area during an Asian dust (Kosa) event
Variations in the structure of airborne bacterial communities in a downwind area during an Asian dust (Kosa) event
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DOI：
10.1016/j.scitotenv.2014.04.044
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发表时间：
2014-08-01
影响因子：
9.8
通讯作者：
Iwasaka, Yasunobu
中科院分区：
文献类型：
--
作者：
Maki, Teruya;Puspitasari, Findya;Iwasaka, Yasunobu
亚洲沙尘（罗莎）事件传播空气中的微生物，显着影响生物生态系统，人类健康，并在顺风地区的冰云形成。然而，空气中的细菌的组成和种群动态很少被调查在下风向地区在科萨事件。在这项研究中，空气采样顺序进行了罗莎事件到达区（日本金泽市）内的10米高的建筑物的顶部，从2011年5月1日至5月7日，在罗莎事件。在罗莎事件期间，细菌细胞和矿物颗粒的颗粒浓度比非罗莎事件日高10倍。从空气样品制备的16 S核糖体DNA克隆文库主要包含来自三个门的序列：蓝细菌门、厚壁菌门和α变形菌门。蓝细菌的克隆主要来自海洋类型的聚球藻属物种，该物种在Rosa事件的第一阶段占主导地位，并在整个Rosa事件中不断检测到。α-变形菌主要在Rosa事件的初期和末期被检测到，系统发育分析表明它们与海洋细菌分支（SAR分支）和鞘氨醇单胞菌属（Sphingomonaspp.）在Rosa事件中期，厚壁菌门的枯草芽孢杆菌和短小芽孢杆菌占优势;已知这些菌种在中国沙漠上空的大气中占优势，这是Rosa事件期间沙尘的来源。Rosa事件结束后获得的克隆主要来自巨大芽孢杆菌，据认为其起源于当地陆地地区。我们的研究结果表明，空气中的细菌群落在地面水平的罗莎事件影响的地区改变其物种组成在罗莎事件对那些含有陆地和浮游细菌从日本海和中国大陆地区的罗莎事件。(C)2014爱思唯尔有限公司版权所有。
Asian dust (Rosa) events transport airborne microorganisms that significantly impact biological ecosystems, human health, and ice-cloud formation in downwind areas. However, the composition and population dynamics of airborne bacteria have rarely been investigated in downwind areas during Kosa events. In this study, air samplings were sequentially performed at the top of a 10-m high building within the Rosa event arrival area (Kanazawa City, Japan) from May 1 to May 7, 2011, during a Rosa event. The particle concentrations of bacterial cells and mineral particles were ten-fold higher during the Rosa event than on non-Rosa event days. A 16S ribosomal DNA clone library prepared from the air samples primarily contained sequences from three phyla: Cyanobacteria, Firmicutes, and Alphaproteobacteria. The clones from Cyanobacteria were mainly from a marine type of Synechococcus species that was dominant during the first phase of the Rosa event and was continuously detected throughout the Rosa event. The clones from Alphaproteobacteria were mainly detected at the initial and final periods of the Rosa event, and phylogenetic analysis showed that their sequences clustered with those from a marine bacterial clade (the SAR clade) and Sphingomonas spp. During the middle of the Rosa event, the Firmicutes species Bacillus subtilis and Bacillus pumilus were predominant; these species are known to be predominant in the atmosphere above the Chinese desert, which is the source of the dust during Rosa events. The clones obtained after the Rosa event had finished were mainly from Bacillus megaterium, which is thought to originate from local terrestrial areas. Our results suggest that airborne bacterial communities at the ground level in areas affected by Rosa events change their species compositions during a Rosa event toward those containing terrestrial and pelagic bacteria transported from the Sea of Japan and the continental area of China by the Rosa event. (C) 2014 Elsevier B.V. All rights reserved.