Bilirubin as a determinant for altered neurogenesis, neuritogenesis, and synaptogenesis.
Bilirubin as a determinant for altered neurogenesis, neuritogenesis, and synaptogenesis.
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DOI：
10.1002/dneu.20727
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发表时间：
2009-08
影响因子：
3
通讯作者：
Brites, Dora
中科院分区：
文献类型：
--
作者：
Fernandes, Adelaide;Falcao, Ana Sofia;Abranches, Elsa;Bekman, Evguenia;Henrique, Domingos;Lanier, Lorene M.;Brites, Dora
关键词：
出生后最初几周内血清未结合胆红素（UCB）水平升高可能导致长期神经功能损害。我们以前报道过，早期暴露于发育中的神经元脐带血，在模仿中度至重度新生儿黄疸的条件下，导致神经炎性萎缩和细胞死亡。在这里，我们进一步分析了UCB对神经细胞分化和神经元发育的影响，解决了UCB如何影响未分化的神经前体细胞的活力和它们的命运决定，以及海马神经元在树突和轴突伸长和分支，轴突生长锥形态，以及树突棘和突触的建立方面的发育。我们的研究结果表明，UCB减少了增殖的神经前体细胞的活力，减少神经发生而不影响星形胶质细胞的形成，并增加分化细胞的细胞功能障碍。此外，早期暴露于UCB的神经元在体外（DIV）的3和9天的树突和轴突分支的数量减少，并且更高数量的神经元显示出更小的生长锥面积。UCB处理的神经元还显示在21 DIV时树突棘和突触的密度降低。UCB在神经元分化、发育和可塑性中的这种有害作用可能会损害大脑在以后生活中的表现。
Elevated levels of serum unconjugated bilirubin (UCB) in the first weeks of life may lead to long-term neurologic impairment. We previously reported that an early exposure of developing neurons to UCB, in conditions mimicking moderate to severe neonatal jaundice, leads to neuritic atrophy and cell death. Here, we have further analyzed the effect of UCB on nerve cell differentiation and neuronal development, addressing how UCB may affect the viability of undifferentiated neural precursor cells and their fate decisions, as well as the development of hippocampal neurons in terms of dendritic and axonal elongation and branching, the axonal growth cone morphology, and the establishment of dendritic spines and synapses. Our results indicate that UCB reduces the viability of proliferating neural precursors, decreases neurogenesis without affecting astrogliogenesis, and increases cellular dysfunction in differentiating cells. In addition, an early exposure of neurons to UCB decreases the number of dendritic and axonal branches at 3 and 9 days in vitro (DIV), and a higher number of neurons showed a smaller growth cone area. UCB-treated neurons also reveal a decreased density of dendritic spines and synapses at 21 DIV. Such deleterious role of UCB in neuronal differentiation, development, and plasticity may compromise the performance of the brain in later life.
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DOI：
10.1083/jcb.109.4.1621
发表时间：
1989-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goslin K;Birgbauer E;Banker G;Solomon F
通讯作者：
Solomon F
影响因子：
3
作者：
Benitez-King, G.;Ramirez-Rodriguez, G.;Meza, I
通讯作者：
Meza, I
影响因子：
6.1
作者：
Falcao, AS;Fernandes, A;Brites, D
通讯作者：
Brites, D
影响因子：
3.5
作者：
Carlson, Erik S.;Stead, John D. H.;Georgieff, Michael K.
通讯作者：
Georgieff, Michael K.
影响因子：
9.8
作者：
通讯作者：
--