Identification of rCop-1, a new member of the CCN protein family, as a negative regulator for cell transformation.
Identification of rCop-1, a new member of the CCN protein family, as a negative regulator for cell transformation.
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鉴定出 rCop-1（CCN 蛋白家族的新成员）作为细胞转化的负调节因子。
DOI：
10.1128/mcb.18.10.6131
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发表时间：
1998
影响因子：
5.3
通讯作者：
Liang,P
中科院分区：
文献类型：
--
作者：
Zhang,R;Averboukh,L;Zhu,W;Zhang,H;Jo,H;Dempsey,PJ;Coffey,RJ;Pardee,AB;Liang,P
By using a model system for cell transformation mediated by the cooperation of the activated H-rasoncogene and the inactivated p53 tumor suppressor gene,rCop-1was identified by mRNA differential display as a gene whose expression became lost after cell transformation. Homology analysis indicates that rCop-1 belongs to an emerging cysteine-rich growth regulator family called CCN, which includes connective-tissue growth factor, CYR61, CEF10 (v-srcinducible), and the product of thenovproto-oncogene. Unlike the other members of the CCN gene family,rCop-1is not an immediate-early gene, it lacks the conserved C-terminal domain which was shown to confer both growth-stimulating and heparin-binding activities, and its expression is lost in cells transformed by a variety of mechanisms. Ectopic expression ofrCop-1by retroviral gene transfers led to cell death in a transformation-specific manner. These results suggest that rCop-1 represents a new class of CCN family proteins that have functions opposing those of the previously identified members.
