DEFICIENCY IN RHABDOMYOSARCOMAS OF A FACTOR REQUIRED FOR MYOD ACTIVITY AND MYOGENESIS
DEFICIENCY IN RHABDOMYOSARCOMAS OF A FACTOR REQUIRED FOR MYOD ACTIVITY AND MYOGENESIS
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DOI：
10.1126/science.8383879
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发表时间：
1993-03-05
期刊：
影响因子：
56.9
通讯作者：
WEINTRAUB, H
中科院分区：
文献类型：
--
作者：
TAPSCOTT, SJ;THAYER, MJ;WEINTRAUB, H
横纹肌肉瘤细胞表达MyoD家族的成肌螺旋-环-螺旋蛋白，但不分化为骨骼肌细胞。凝胶位移和瞬时转染试验表明，横纹肌肉瘤细胞中的MyoD能够结合DNA，但作为转录激活因子相对无功能。成纤维细胞的异核体形成导致MyoD的转录激活恢复，横纹肌肉瘤细胞分化为骨骼肌细胞。这些结果表明，横纹肌肉瘤缺乏MyoD活性所需的因子。
Rhabdomyosarcoma cells express the myogenic helix-loop-helix proteins of the MyoD family but do not differentiate into skeletal muscle cells. Gel shift and transient transfection assays revealed that MyoD in the rhabdomyosarcoma cells was capable of binding DNA but was relatively nonfunctional as a transcriptional activator. Heterokaryon formation with fibroblasts resulted in the restoration of transcriptional activation by MyoD and the differentiation of the rhabdomyosarcoma cells into skeletal muscle cells. These results suggest that rhabdomyosarcomas are deficient in a factor required for MyoD activity.