Testicular denervation in prepuberty rat inhibits seminiferous tubule development and spermatogenesis.
Testicular denervation in prepuberty rat inhibits seminiferous tubule development and spermatogenesis.
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DOI：
10.1262/jrd.10-009n
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发表时间：
2010-08
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影响因子：
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通讯作者：
Shuying Huo;Zhihao Xu;Xiaoxin Zhang;Jianfang Zhang;S. Cui
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作者：
Shuying Huo;Zhihao Xu;Xiaoxin Zhang;Jianfang Zhang;S. Cui
在成年大鼠中，除内分泌控制机制外，精精上神经（SSN）和精精下神经（ISN）还参与调节睾酮分泌和精子发生。然而，目前关于睾丸神经供应如何调节睾丸发育及其相关机制的数据很少。本研究旨在探讨睾丸神经供应对大鼠青春期前至成年期睾丸成熟、精子发生的调节作用及其机制。我们在产后30天对大鼠的ssn和isn进行了横切，分析了PD60和PD90时睾丸形态和附睾尾精子含量、细胞增殖和凋亡以及原代精子细胞减数分裂的变化。结果表明，睾丸去神经支配可显著降低睾丸质量、附睾尾部精子数量和血清睾酮浓度。增殖细胞核抗原（PCNA）和裂解caspase-3免疫组化染色证实去神经支配对原代精原细胞和精原细胞的增殖无影响，但明显促进圆形精母细胞和间质细胞的凋亡。这是一个新的发现，去神经支配通过突触复合体蛋白3 （SCP3）的表达减少了zygotene和pachytene精母细胞的减数分裂激活-减数分裂的标志。RT-PCR结果显示，去神经支配可显著降低睾丸3β -羟基类固醇脱氢酶1 （3β - hsd1）和促黄体生成素受体（LHR） mRNA水平，但对睾丸促卵泡激素受体（FSHR） mRNA表达无明显影响。提示睾丸神经供应在青春期前至成年期精管发育和精子发生中起重要作用。
In the adult rat, the superior spermatic nerve (SSN) and inferior spermatic nerve (ISN) are involved in regulating testosterone secretion and spermatogenesis, in addition to endocrine control mechanisms. However, there are currently few data on how the testis nerve supply regulates testicular development and related mechanisms. The present study was thus designed to investigate the regulating effects of testis nerve supply to testicular maturation, spermatogenesis and the involved mechanisms from prepuberty to adulthood in rats. We transected the SSNs and ISNs of rats on postnatal day (PD) 30 and then analyzed changes in testicular morphology and cauda epididymal sperm content, cell proliferation and apoptosis and primary spermatocyte meiosis on PD60 and PD90. The results demonstrated that testicular denervation significantly reduced testis mass, cauda epididymal sperm counts and serum testosterone concentrations. Proliferating cell nuclear antigen (PCNA) and cleaved caspase-3 immunohistochemistry staining proved that the denervation had no influence on the proliferation of spermatogonia and primary spermatocytes, but obviously promoted the apoptosis of round spermatids and Leydig cells. It is novel that denervation reduced the meiotic activation of zygotene and pachytene spermatocytes through the expression of synaptonemal complex protein 3 (SCP3)-a marker of meiosis. In addition, RT-PCR showed that testis denervation significantly decreased testis 3beta-hydroxysteroid dehydrogenase 1 (3beta-HSD1) and luteinizing hormone receptor (LHR) mRNA levels, but had no obvious influence on testis follicle stimulating hormone receptor (FSHR) mRNA expression. These results suggest that the testicular nerve supply plays an important role in supporting seminiferous tubule development and spermatogenesis from prepuberty to adulthood.