Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles.
Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles.
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DOI：
10.1002/jbm.b.30443
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发表时间：
2006-05
期刊：
影响因子：
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通讯作者：
K. Shimizu;A. Ito;H. Honda
中科院分区：
文献类型：
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作者：
K. Shimizu;A. Ito;H. Honda
为了设计功能性组织，必须将大量细胞成功地接种到支架中。我们以前提出了一种使用磁铁矿纳米颗粒和磁力的组织工程方法，我们称之为“Mag-TE”。在本研究中，我们将Mag-TE技术应用于细胞接种过程，并将该技术称为“Mag-seeding”。使用具有各种孔径的六种类型的市售支架（5种胶原海绵和1种D、D-L、L聚乳酸海绵）研究了通过Mag-seeding的NIH/3 T3成纤维细胞（FB）的细胞接种效率。用我们的原始磁铁矿阳离子脂质体（MCL）磁性标记FB，该脂质体具有正表面电荷，以改善在细胞表面上的吸附。将标记有MCL的FB接种到支架上，并将磁体（4kG）放置在支架下。磁接种促进成功的细胞接种到深的内部空间的支架。细胞接种效率显着增加，在所有的支架相比，没有磁力。此外，当使用高强度磁体（10 kG）时，细胞接种效率显著提高。这些结果表明，磁接种是一种很有前途的组织工程方法。
To engineer functional tissues, a large number of cells must be successfully seeded into scaffolds. We previously proposed a methodology for tissue engineering using magnetite nanoparticles and magnetic force, which we termed "Mag-TE." In the present study, we applied the Mag-TE technique to a cell seeding process and have termed the technique "Mag-seeding." The cell-seeding efficiency of NIH/3T3 fibroblasts (FBs) by Mag-seeding was investigated using six types of commercially available scaffolds (5 collagen sponges and 1 D,D-L,L polylactic acid sponge) having various pore sizes. FBs were magnetically labeled with our original magnetite cationic liposomes (MCLs), which have a positive surface charge, to improve adsorption onto the cell surface. FBs labeled with MCLs were seeded onto a scaffold, and a magnet (4 kG) was placed under the scaffold. Mag-seeding facilitated successful cell seeding into the deep internal space of the scaffolds. Cell-seeding efficiency increased significantly in all scaffolds when compared to those without magnetic force. Moreover, when a high-intensity magnet (10 kG) was used, cell-seeding efficiency was significantly enhanced. These results suggest that Mag-seeding is a promising approach for tissue engineering.