Immunosuppressive activity of daphnetin, one of coumarin derivatives, is mediated through suppression of NF-κB and NFAT signaling pathways in mouse T cells.
Immunosuppressive activity of daphnetin, one of coumarin derivatives, is mediated through suppression of NF-κB and NFAT signaling pathways in mouse T cells.
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DOI：
10.1371/journal.pone.0096502
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Guan S
中科院分区：
文献类型：
--
作者：
Song B;Wang Z;Liu Y;Xu S;Huang G;Xiong Y;Zhang S;Xu L;Deng X;Guan S
瑞香素是一种从植物中提取的有效化合物，是香豆素的二羟基化衍生物。香豆素衍生物具有抗血栓、抗炎和抗氧化活性。本研究旨在探讨瑞香素对刀豆蛋白A（ConA）诱导的小鼠T淋巴细胞免疫抑制作用及其机制。我们发现，在体外，瑞香素抑制ConA诱导的脾细胞增殖，影响细胞因子的产生，并通过G 0/G1期转换抑制细胞周期进程。瑞香素可下调ConA诱导的小鼠T淋巴细胞NF-κB和NFAT信号转导通路的活化。瑞香素对2，4-二硝基氟苯（DNFB）诱发的小鼠迟发型超敏反应有明显的抑制作用。瑞香素在体内外均具有较强的免疫抑制活性，提示瑞香素具有潜在的免疫抑制作用，为进一步研究瑞香素奠定了基础。
Daphnetin, a plant-derived dihydroxylated derivative of coumarin, is an effective compound extracted from a plant called Daphne Korean Nakai. Coumarin derivates were known for their antithrombotic, anti-inflammatory, and antioxidant activities. The present study was aimed to determine the immunosuppressive effects and the underlying mechanisms of daphnetin on concanavalin A (ConA) induced T lymphocytes in mice. We showed that, in vitro, daphnetin suppressed ConA-induced splenocyte proliferation, influenced production of the cytokines and inhibited cell cycle progression through the G0/G1 transition. The data also revealed that daphnetin could down-regulate activation of ConA induced NF-κB and NFAT signal transduction pathways in mouse T lymphocyte. In vivo, daphnetin treatment significantly inhibited the 2, 4- dinitrofluorobenzene (DNFB) -induced delayed type hypersensitivity (DTH) reactions in mice. Collectively, daphnetin had strong immunosuppressive activity both in vitro and in vivo, suggesting a potential role for daphnetin as an immunosuppressive agent, and established the groundwork for further research on daphnetin.
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