Isolation of Bacillus spp. with High Fibrinolytic Activity and Performance Evaluation in Fermented Douchi
Isolation of Bacillus spp. with High Fibrinolytic Activity and Performance Evaluation in Fermented Douchi
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高纤溶活性芽孢杆菌的分离及发酵豆豉性能评价
DOI：
10.4315/jfp-20-307
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发表时间：
2021-04-01
影响因子：
2
通讯作者：
Zeng, Xuefeng
中科院分区：
文献类型：
--
作者：
Long, Jia;Zhang, Xin;Zeng, Xuefeng
纤溶酶治疗心脑血管疾病疗效确切，安全性高。因此，筛选具有优良发酵性能的产纤溶酶微生物菌株具有重要的工业应用价值。采用纤维蛋白平板法从不同发酵食品中筛选纤溶酶高产菌株，并对筛选出的菌株进行分子生物学初步鉴定。然后，将这些菌株用于大豆的固态发酵。并对发酵产物豆豉进行纤溶活性测定、感官评定和生物胺含量测定。通过主成分分析法综合评价各菌株的发酵性能。最后，根据菌株形态、生理生化特性、16 S rDNA序列和系统发育分析结果对目的菌株进行了鉴定。共筛选出15株纤溶酶活性较高的芽孢杆菌。它们的纤溶酶产生活性水平高于5，500 IU/g。通过分子生物学分析，共分离到4株枯草芽孢杆菌、10株解淀粉芽孢杆菌和1株velezensis芽孢杆菌。主成分分析结果表明，SN-14的发酵性能最好，降低了组胺和总胺的积累，发酵豆豉的纤溶活性达到5920.5 ± 107.7 IU/g，感官评分为4.6 ± 0.3（满分5分）。综合生理生化分析结果，SN-14为Velezensis芽孢杆菌。高产纤溶酶且发酵性能优良的Bacillus velezensis SN-14具有潜在的工业应用价值。
Fibrinolytic enzymes are effective and highly safe in treating cardiovascular and cerebrovascular diseases. Therefore, screening fibrinolytic enzyme-producing microbial strains with excellent fermentation performance is of great value to industrial applications. The fibrin plate method was used in screening strains with high yields of fibrinolytic enzymes from different fermented food products, and the screened strains were preliminarily identified using molecular biology. Then, the strains were used for solid-state fermentation of soybeans. Moreover, the fermentation product douchi was subjected to fibrinolytic activity measurement, sensory evaluation, and biogenic amine content determination. The fermentation performance of each strain was comprehensively evaluated through principal component analysis. Finally, the target strain was identified based on strain morphology, physiological and biochemical characteristics, 16S rDNA sequence, and phylogenetic analysis results. A total of 15 Bacillus species with high fibrinolysin activity were selected. Their fibrinolytic enzyme-producing activity levels were higher than 5,500 IU/g. Through molecular biology analysis, we found 4 strains of Bacillus subtilis, 10 strains of Bacillus amyloliquefaciens, and 1 strain of Bacillus velezensis. The principal component analysis results showed that SN-14 had the best fermentation performance and reduced the accumulation of histamine and total amine, the fibrinolytic activity of fermented douchi reached 5,920.5 +/- 107.7 IU/g, and the sensory score was 4.6 +/- 0.3 (out of 5 points). Finally, the combined results of physiological and biochemical analyses showed SN-14 was Bacillus velezensis. The high-yield fibrinolytic and excellent fermentation performance strain Bacillus velezensis SN-14 has potential industrial application.