The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes.
The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes.
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小鼠雄性生殖细胞特异性基因 Tpx-1：分子结构、精子发生中的表达模式以及与两个非哺乳动物基因的序列相似性。
DOI：
10.1007/bf00292155
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kasahara,M
中科院分区：
文献类型：
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作者：
Mizuki,N;Sarapata,DE;Garcia-Sanz,JA;Kasahara,M
Tpx-1是定位于小鼠第17号染色体上的睾丸特异基因。推测的TPX-1蛋白与酸性附睾糖蛋白（AEG）具有55%的氨基酸序列相似性，推测AEG参与精子成熟。在本研究中，我们确定了小鼠Tpx-1基因的基因组结构及其转录本的细胞定位。该基因被发现包含10个外显子，在外显子8和9之间有一个异常大的内含子（17.0个内切酶对）。睾丸切片原位杂交结果表明，Tpx-1在单倍体雄性生殖细胞中大量转录。蛋白质数据库的计算机搜索显示，推导的TPX-1/AEG蛋白有显着的序列相似性（约30%）的两个非哺乳动物的蛋白质：“发病机制相关”的蛋白质1的烟草，和毒囊蛋白的白面黄蜂，被称为Dol m V。氨基酸残基编码的外显子10的TPX-1基因和大部分编码的外显子9的非哺乳动物的蛋白质是不存在的。这一结果表明，Tpx-1的祖先获得外显子9和10后，从植物和昆虫蛋白质的祖先分歧。
Tpx-1is a testis-specific gene that maps on mouse Chromosome (Chr) 17. The deduced TPX-1 protein shows 55% amino acid sequence similarity to acidic epididymal glycoprotein (AEG), assumed to be involved in sperm maturation. In the present study, we determined the genomic structure of the mouseTpx-1gene and the cellular localization of its transcripts. The gene was found to contain ten exons, with an unusually large intron (∼17.0 kilobase pairs) between exons 8 and 9. In situ hybridization of testicular sections showed thatTpx-1is transcribed abundantly by haploid male germ cells. A computer search of protein databases revealed that deduced TPX-1/AEG proteins have significant sequence similarity (∼30%) to two non-mammalian proteins: “pathogenesis-related” proteins 1 of tobaccos, and venom sac proteins of whiteface hornets, known as Dol m V. Amino acid residues encoded by exon 10 of theTpx-1gene and most of those encoded by exon 9 were absent in the non-mammalian proteins. This result suggests that the ancestor ofTpx-1acquired exons 9 and 10 after its divergence from the ancestors of the plant and insect proteins.