The Immunohistochemical Demonstration of Major Histocompatibility Antigens in the Human Kidney Using Monoclonal Antibodies
The Immunohistochemical Demonstration of Major Histocompatibility Antigens in the Human Kidney Using Monoclonal Antibodies
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使用单克隆抗体对人肾中主要组织相容性抗原进行免疫组织化学论证
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Atkins
中科院分区：
文献类型：
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作者：
W. Hancock;N. Kraft;R. Atkins
利用单克隆抗体和冷冻切片和石蜡切片的4层免疫过氧化物酶技术，研究了HLA - ABC和DR抗原在人肾脏中的分布。酶切石蜡切片提供了最好的定位。HLA‐ABC抗原位于所有肾内皮、鲍曼囊和近端小管。HLA‐DR抗原局限于毛细血管和静脉内皮以及近端小管的低密度。不同的单克隆抗体在树突状细胞的分布上发现了微小的差异，但使用HLA - ABC、HLA - DR或白细胞-常见抗原的单克隆抗体未检测到树突状细胞。
Summary The distribution of HLA‐ABC and DR antigens in human kidneys was studied using monoclonal antibodies to mono‐morphic determinants and a 4‐layer immunoperoxidase technique applied to frozen and paraffin sections. Enzyme digested paraffin sections provided the best localization. HLA‐ABC antigens were located on all renal endothelium, Bowman's capsule and in proximal tubules. HLA‐DR antigen was restricted to capillary and venous endothelium plus, at low density, in proximal tubules. Minor differences in distribution were found with different monoclonal antibodies, but dendritic cells were not detected using monoclonal antibodies to HLA‐ABC, HLA‐DR or leukocyte‐common antigens.