Hemagglutinin linear epitope presentation on monolayer-protected clusters elicits strong antibody binding
Hemagglutinin linear epitope presentation on monolayer-protected clusters elicits strong antibody binding
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DOI：
10.1021/bm050475o
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发表时间：
2005-11-01
影响因子：
6.2
通讯作者：
Cliffel, DE
中科院分区：
文献类型：
--
作者：
Gerdon, AE;Wright, DW;Cliffel, DE
Immunoreactive, multicomponent nanoclusters were assembled through the controlled presentation of a known, synthetic peptide epitope. The epitope comes from the hemagglutanin protein of influenza and is known to bind to a monoclonal anti-HA antibody. Antibody affinity for the immunoreactive MPC was compared to the affinity for traditionally used peptide arrays using the quartz crystal microbalance. The two systems had comparable affinities (K-a), ranging from 0.41 x 10(7) M-1 to 1.8 x 10(7) M-1, though the nanocluster used a much lower density of peptide relative to that of the peptide array. These results suggest that functionalized nanoclusters have potential in nanostructure assembly and medical applications. Water-soluble nanoparticles that present known neutralizing peptide epitopes of protein antigens might be used in antiviral influenza vaccines.
