A new kind of informational suppression in the nematode Caenorhabditis elegans.
A new kind of informational suppression in the nematode Caenorhabditis elegans.
复制标题
线虫秀丽隐杆线虫中的一种新型信息抑制。
DOI：
10.1093/genetics/123.2.301
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Anderson,P
中科院分区：
文献类型：
--
作者：
Hodgkin,J;Papp,A;Pulak,R;Ambros,V;Anderson,P
影响三个不同的秀丽隐杆线虫基因的突变的独立逆转各自产生了同一组基因外抑制子的代表。六个位点中任何一个的突变都可以作为unc-54（肌球蛋白重链基因）、lin-29（异时基因）和tra-2（性别决定基因）某些等位基因的等位基因特异性隐性抑制基因。同样的突变也抑制了另一个性别决定基因tra-1和形态发生基因dpy-5的某些等位基因。除了它们的抑制表型外，抑制基因突变还引起雄性腔上囊和雌雄同体外阴的异常形态发生。我们将这些基因命名为smg-1到smg-6（对生殖器有形态发生作用的抑制基因），以区别于可以突变产生异常生殖器但不起抑制作用的mab（男性异常）基因（smg-1和smg-2是先前描述的两个基因mab-1和mab-11的新名称）。抑制的模式，和不同的smg基因之间的相互作用，进行了描述和讨论。一般来说，抑制是隐性的和不完全的，并且至少一些被抑制的突变本质上是亚晶型的。unc-54的可抑制等位基因在基因的3'非编码区含有缺失;基因的蛋白质编码区显然不受影响。这表明smg抑制子影响翻译以外的过程，例如mRNA加工，运输或稳定性。
Independent reversions of mutations affecting three different Caenorhabditis elegans genes have each yielded representatives of the same set of extragenic suppressors. Mutations at any one of six loci act as allele-specific recessive suppressors of certain allels of unc-54 (a myosin heavy chain gene), lin-29 (a heterochronic gene), and tra-2 (a sex determination gene). The same mutations also suppress certain alleles of another sex determination gene, tra-1, and of a morphogenetic gene, dpy-5. In addition to their suppression phenotype, the suppressor mutations cause abnormal morphogenesis of the male bursa and the hermaphrodite vulva. We name these genes smg-1 through smg-6 (suppressor with morphogenetic effect on genitalia), in order to distinguish them from mab (male abnormal) genes that can mutate to produce abnormal genitalia but which do not act as suppressors (smg-1 and smg-2 are new names for two previously described genes, mab-1 and mab-11). The patterns of suppression, and the interactions between the different smg genes, are described and discussed. In general, suppression is recessive and incomplete, and at least some of the suppressed mutations are hypomorphic in nature. A suppressible allele of unc-54 contains a deletion in the 3' noncoding region of the gene; the protein coding region of the gene is apparently unaffected. This suggests that the smg suppressors affect a process other than translation, for example mRNA processing, transport, or stability.