Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs.

Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs.
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DOI:
10.1007/978-1-62703-125-7_10
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发表时间:
2013
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Dobbs, Leland G
Dobbs, Leland G
中科院分区:
其他
文献类型:
--
作者:
Gonzalez, Robert F;Dobbs, Leland G

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肺泡上皮由肺泡I型(TI)和II型(TII)细胞组成,覆盖了肺内表面积的99%以上。对分离和培养的肺泡上皮TI和TII细胞的研究提供了关于这两种细胞类型功能的大量信息。本章提供了从大鼠肺中分离和培养这两种细胞类型的方法的信息。
The pulmonary alveolar epithelium, comprised of alveolar Type I (TI) and Type II (TII) cells, covers more than 99% of the internal surface area of the lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.