Detection of an IS21 insertion sequence in the mexR gene of Pseudomonas aeruginosa increasing β-lactam resistance
Detection of an IS21 insertion sequence in the mexR gene of Pseudomonas aeruginosa increasing β-lactam resistance
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DOI：
10.1016/s0378-1097(03)00882-6
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发表时间：
2004-01-15
影响因子：
2.1
通讯作者：
Reynaud, A
中科院分区：
文献类型：
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作者：
Boutoille, D;Corvec, S;Reynaud, A
为了了解对替卡西林和氨曲南敏感性降低的铜绿假单胞菌临床菌株中MexAB OprM外排系统的调节，通过聚合酶链反应（PCR）扩增mexR阻遏物基因，并显示其被超过2 kb的插入序列破坏，具有特征性的直接和反向重复序列。测序结果表明，该基因含有2131 bp的IS21插入序列。逆转录PCR方法被用来量化mexA的成绩单，并显示在这个菌株的mexA的转录率增加，与PAO1对照菌株。铜绿假单胞菌中的nalB表型可能是由于点突变，但也可能是由于mexR调节基因中存在插入序列。(C)2003年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
To understand the regulation of the MexAB OprM efflux system in a clinical strain of Pseudomonas aeruginosa presenting a decreased susceptibility to ticarcillin and aztreonam, the mexR repressor gene was amplified by polymerase chain reaction (PCR) and was shown to be disrupted by an insertion sequence of more than 2 kb, with characteristic direct and inverted repeat sequences. Sequencing revealed a 2131-bp IS21 insertion sequence. A reverse transcription PCR method was used to quantify mexA transcripts and showed an increased transcription rate of mexA in this strain, compared with a PAO1 control strain. The nalB phenotype in P. aeruginosa may be due to point mutations, but also to the presence of an insertion sequence in the mexR regulator gene. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.