Shared and distinct functions of two Gti1/Pac2 family proteins in growth, morphogenesis and pathogenicity of Magnaporthe oryzae
Shared and distinct functions of two Gti1/Pac2 family proteins in growth, morphogenesis and pathogenicity of Magnaporthe oryzae
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DOI：
10.1111/1462-2920.12204
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发表时间：
2014-03-01
影响因子：
5.1
通讯作者：
Zhang, Zhengguang
中科院分区：
文献类型：
--
作者：
Chen, Yue;Zhai, Su;Zhang, Zhengguang
Gti 1/Pac 2是一类保守的家族蛋白，在粟酒裂殖酵母和白色念珠菌等酵母菌中调节形态发生转变，在禾谷镰刀菌中也控制毒素的产生和致病性。为了测试Gti 1/Pac 2旁系同源物MoGti 1和MoPac 2在稻瘟病菌中的功能，我们分别产生了Mogti 1和Mopac 2突变株。我们发现MoGti 1和MoPac 2在菌丝生长、分生孢子形成、有性繁殖、胁迫反应、表面疏水性、侵入性菌丝生长和致病性中表现出共同和不同的作用。与MoGti 1的保守功能一致，我们发现MoGti 1-GFP定位于细胞核，而MoPac 2-GFP主要存在于细胞质中。此外，我们提供的证据表明，MoGti 1的核定位可以受到MoPmk 1丝裂原活化蛋白激酶的调节。此外，我们发现Mopac 2突变体的致病性降低与植物防御基因的表达增加相对应，包括PR 1a，AOS 2，LOX 1，PAD 4和CHT 1。综上所述，我们的研究提供了一个全面的分析，两个相似的，但不同的Gti 1/Pac 2家族的蛋白质在M.的，这强调了重要的，但保守的功能，这些家庭的蛋白质在植物病原真菌。
Gti1/Pac2 are conserved family proteins that regulate morphogenic transition in yeasts such as Schizosaccharomyces pombe and Candida albicans, and they also control toxin production and pathogenicity in filamentous fungus Fusarium graminearum. To test the functions of Gti1/Pac2 paralogues MoGti1 and MoPac2 in the rice blast fungus Magnaporthe oryzae, we generated respective Mogti1 and Mopac2 mutant strains. We found that MoGti1 and MoPac2 exhibit shared and distinct roles in hyphal growth, conidiation, sexual reproduction, stress responses, surface hydrophobility, invasive hyphal growth and pathogenicity. Consistent with the putative conserved function of MoGti1, we showed that MoGti1-GFP is localized to the nucleus, whereas MoPac2-GFP is mainly found in the cytoplasm. In addition, we provided evidence that the nuclear localization of MoGti1 could be subject to regulation by MoPmk1 mitogen-activated protein kinase. Moreover, we found that the reduced pathogenicity in the Mopac2 mutant corresponds with an increased expression of plant defence genes, including PR1a, AOS2, LOX1, PAD4, and CHT1. Taken together, our studies provide a comprehensive analysis of two similar but distinct Gti1/Pac2 family proteins in M.oryzae, which underlines the important yet conserved functions of these family proteins in plant pathogenic fungi.