DNA vaccination using coexpression of cytokine genes with a bacterial gene encoding a 60-kDa heat shock protein
DNA vaccination using coexpression of cytokine genes with a bacterial gene encoding a 60-kDa heat shock protein
复制标题
使用细胞因子基因与编码 60 kDa 热休克蛋白的细菌基因共表达进行 DNA 疫苗接种
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
I. Autenrieth
中科院分区：
文献类型：
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作者：
M. Hornef;A. Noll;R. Schirmbeck;J. Reimann;I. Autenrieth
Abstract Coexpression of cytokine genes together with antigen-encoding genes in DNA vaccination vectors can increase humoral and cellular immune responses and may steer them in a Th1 or Th2 direction. In this study, the modulatory effect of interleukin (IL)-2, IL-4, and interferon (IFN)-γ coexpressed with the 60-kDa heat shock protein (Hsp60) of Yersinia enterocolitica O:8 (Y-Hsp60) was studied. DNA vaccination with y-hsp60 evoked specific humoral and cellular immune responses as well as reduction of the splenic bacterial load upon challenge with Y. enterocolitica in a mouse infection model. Coexpression of IL-2 or IFN-γ enhanced Y. enterocolitica-specific total IgG (P < 0.05) and IgG2a antibody responses. Coexpression of IFN-γ also improved the proliferative T cell responses upon stimulation with Y-Hsp60. A reduction of the splenic bacterial load as compared with the plasmid encoding Y-Hsp60 only was found for the IFN-γ coexpressing vector. Thus, coexpression of cytokine genes such as IFN-γ in DNA vaccination vectors might improve immunity and help to overcome the side effects of standard adjuvants.
