Cell-Cycle Independent Chromosome Condensation in Schizosaccharomyces pombe Induced by High Hydrostatic Pressure Treatment
Cell-Cycle Independent Chromosome Condensation in Schizosaccharomyces pombe Induced by High Hydrostatic Pressure Treatment
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高静水压处理诱导粟酒裂殖酵母中细胞周期独立的染色体缩合
DOI：
10.1271/bbb.90118
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
M. Yamasaki
中科院分区：
文献类型：
--
作者：
Seisuke Arai;T. Kawarai;R. Arai;Minoru Yoshida;S. Furukawa;H. Ogihara;M. Yamasaki
我们将粟酒裂殖酵母在28 °C下暴露于75 MPa的高静水压处理（HPT）30分钟，然后观察到DAPI染色的染色体DNA已经紧密地收缩。我们称这种现象为HPT诱导的染色体凝聚（HPT-CC）。HPT没有显著降低生存力。当HPT细胞在28 °C下培养30 min时，凝聚状态被释放。凝聚不是由核膜收缩引起的，这是由YFP标记的importin α可视化的。HPT-CC是细胞周期无关的，因为它被观察到在几乎所有的随机培养的细胞。缩合素复合物（Cut 3、Cut 14和其他三种蛋白质）负责细胞周期依赖性CC。利用Cut 3-YFP和Cut 3和Cut 14的ts突变体的研究证实了HPT-CC不依赖于缩合素分子。在酿酒酵母中也观察到HPT-CC。HPT-CC似乎可能是一个暂时的应力响应高静水压力，至少在酵母中发现。
We exposed Schizosaccharomyces pombe to high hydrostatic pressure treatment (HPT) of 75 MPa at 28 °C for 30 min and then observed that the DAPI-stained chromosomal DNA had shrunk compactly. We termed this phenomenon HPT-induced chromosome condensation (HPT-CC). HPT did not significantly decrease viability. The condensed state was released when HPT cells were cultured at 28 °C for 30 min. The condensation was not caused by shrinking of the nuclear envelope, which was visualized by YFP-tagged importin α. HPT-CC was cell cycle independent, because it was observed in almost all randomly cultured cells. The condensin complex (Cut3, Cut14, and three other proteins) is responsible for cell cycle dependent CC. Studies with Cut3-YFP and ts mutants of Cut3 and Cut14 confirmed that HPT-CC was independent of condensin molecules. HPT-CC was also observed in Saccharomyces cerevisiae. HPT-CC appears likely to be a temporal stress response to high hydrostatic pressure found at least in yeasts.
影响因子：
5
作者：
Kawarai, T;Wachi, M;Yamasaki, M
通讯作者：
Yamasaki, M