Transcriptome Comparison of Global Distinctive Features Between Pollination and Parthenocarpic Fruit Set Reveals Transcriptional Phytohormone Cross-Talk in Cucumber (Cucumis sativus L.)
Transcriptome Comparison of Global Distinctive Features Between Pollination and Parthenocarpic Fruit Set Reveals Transcriptional Phytohormone Cross-Talk in Cucumber (Cucumis sativus L.)
复制标题
授粉和单性结实之间整体特征的转录组比较揭示了黄瓜（Cucumis sativus L.）中的转录植物激素交叉对话
DOI：
10.1093/pcp/pcu051
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发表时间：
2014-07-01
影响因子：
4.9
通讯作者：
Chen, Jinfeng
中科院分区：
文献类型：
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作者：
Li, Ji;Wu, Zhe;Chen, Jinfeng
单性结实是决定果树产量和品质的重要性状。然而，单性结实诱导的机制的理解是有限的。黄瓜（Cucumis sativus L）具有丰富的单性结实种质资源，是研究单性结实的理想模式生物。在这项研究中，黄瓜果实的转录组进行了研究，使用RNA测序（RNA-Seq）。将坐果的差异表达基因（DEG）与败育果进行比较。将转录组分析与细胞形态学和生理学分析相结合，揭示了单性结实和授粉果实的显著特征。单性结实果实的细胞分裂和细胞分裂基因的转录都比较活跃。该研究还表明，单性结实坐果是一个高糖消耗过程，其通过导致碳水化合物分解的基因转录增强碳水化合物降解来实现。此外，这项工作提供的证据支持单性结实坐果是通过在转录水平上模拟授粉/受精过程诱导的，即通过执行与授粉果实中诱导授粉和配子体发育的基因相同的转录模式。根据RNA-Seq和卵巢瞬时表达结果，预测14个基因为推定的单性结实基因。对这些候选基因的转录分析揭示了单性结实坐果过程中生长素、细胞分裂素和赤霉素在转录水平上的相互作用。
Parthenocarpy is an important trait determining yield and quality of fruit crops. However, the understanding of the mechanisms underlying parthenocarpy induction is limited. Cucumber (Cucumis sativus L) is abundant in parthenocarpic germplasm resources and is an excellent model organism for parthenocarpy studies. In this study, the transcriptome of cucumber fruits was studied using RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) of set fruits were compared against aborted fruits. Distinctive features of parthenocarpic and pollinated fruits were revealed by combining the analysis of the transcriptome together with cytomorphological and physiological analysis. Cell division and the transcription of cell division genes were found to be more active in parthenocarpic fruit. The study also indicated that parthenocarpic fruit set is a high sugar-consuming process which is achieved via enhanced carbohydrate degradation through transcription of genes that lead to the breakdown of carbohydrates. Furthermore, the evidence provided by this work supports a hypothesis that parthenocarpic fruit set is induced by mimicking the processes of pollination/fertilization at the transcriptional level, i.e. by performing the same transcriptional patterns of genes inducing pollination and gametophyte development as in pollinated fruit. Based on the RNA-Seq and ovary transient expression results, 14 genes were predicted as putative parthenocarpic genes. The transcription analysis of these candidate genes revealed auxin, cytokinin and gibberellin cross-talk at the transcriptional level during parthenocarpic fruit set.