Differential Effects of Soy Isoflavones on the Biophysical Properties of Model Membranes
Differential Effects of Soy Isoflavones on the Biophysical Properties of Model Membranes
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DOI：
10.1021/acs.jpcb.3c08390
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发表时间：
2024-02-28
影响因子：
3.3
通讯作者：
Lee，Sunghee
中科院分区：
文献类型：
--
作者：
Gudyka，Jamie;Ceja-Vega，Jasmin;Lee，Sunghee
用透膜透水率、差示扫描量热法和共聚焦拉曼光谱研究了主要大豆异黄酮染料木素和大豆苷元对1，2-二油酰甘油-3-磷胆碱(DOPC)或DOPC与胆固醇(摩尔比为4：1)模型脂双层生物物理性质的影响。随着染料木素浓度的增加，膜的透水性增加，相变温度降低，相变热降低，膜内堆积无序。相反，在可比浓度下，大豆苷元降低透水性，对热力学参数和堆积无序的影响可以忽略不计。对于含胆固醇的DOPC双层膜，染料木素和大豆苷元对透膜透水性的影响总体上不那么明显。它们各自具有不同的能力来改变具有不同脂组成的生物膜的物理和结构性质，这意味着对异黄酮相互作用的复杂和敏感的性质，这取决于双层堆积的初始状态和这些大豆异黄酮的分子结构的差异，并为理解这些分子与细胞膜的相互作用提供了见解。
The effects that the main soy isoflavones, genistein and daidzein, have upon the biophysical properties of a model lipid bilayer composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or DOPC with cholesterol (4 to 1 mol ratio) have been investigated by transbilayer water permeability, differential scanning calorimetry, and confocal Raman microspectroscopy. Genistein is found to increase water permeability, decrease phase transition temperature, reduce enthalpy of transition, and induce packing disorder in the DOPC membrane with an increasing concentration. On the contrary, daidzein decreases water permeability and shows negligible impact on thermodynamic parameters and packing disorder at comparable concentrations. For a cholesterol-containing DOPC bilayer, both genistein and daidzein exhibit an overall less pronounced effect on transbilayer water permeability. Their respective differential abilities to modify the physical and structural properties of biomembranes with varying lipid compositions signify a complex and sensitive nature to isoflavone interactions, which depends on the initial state of bilayer packing and the differences in the molecular structures of these soy isoflavones, and provide insights in understanding the interactions of these molecules with cellular membranes.