Developmental expression pattern of monoamine oxidases in sensory organs and neural crest derivatives.
Developmental expression pattern of monoamine oxidases in sensory organs and neural crest derivatives.
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感觉器官和神经嵴衍生物中单胺氧化酶的发育表达模式。
DOI：
10.1002/cne.10804
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Cases,Olivier
中科院分区：
文献类型：
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作者：
Vitalis,Tania;Alvarez,Chantal;Chen,Kevin;Shih,JeanC;Gaspar,Patricia;Cases,Olivier
5-羟色胺（5-HT）已被证明是颅面和心脏发育以及神经嵴衍生物迁移中的形态原。这些结构中的一些能够在发育过程中捕获5-HT，但对这些发育组织中主要单胺降解酶单胺氧化酶（MAO）A和B的定位一无所知。我们产生了一个高度特异性的抗体MAOB，免疫反应性完全废除在大脑提取物或大脑部分缺乏MAOB的小鼠。从使用这种抗体和特定的核糖核酸探针，我们报告说，MAOB表达早期在各种神经嵴衍生物，在面部感觉器官，并在心脏。从E11.5到P0，发现MAOB在以下神经嵴衍生物中强烈表达：主动脉、颅间充质（发育中的骨骼、颅神经节的感觉神经元、软骨、甲状腺和横纹肌）、牙齿间充质、几种软腭衍生物和边界帽细胞（E11.5-P4）。边界帽细胞有助于形成中枢和外周神经系统之间的神经出口-入口点。几个面部感觉器官也含有MAOB mRNA，蛋白质和活性。MAOB在嗅基板、嗅上皮背侧部、嗅神经层（可能是成鞘胶质细胞）、耳蜗神经节细胞、味蕾和触须滤泡中的默克尔细胞中高表达。最后，我们发现MAOB在咽器官、心脏、肝脏和肥大细胞中大量表达。相反，MAOA表达仅限于交感神经节和脑膜和毛细血管。MAOB的表达模式通常与先前报道的血浆5-HT转运蛋白表达或组胺生物合成酶L-组氨酸脱羧酶的表达模式相匹配，表明MAOB在发育胚胎中精细调节5-HT和组胺水平中的作用。J. Comp.神经元464：392-403，2003.© 2003 Wiley利斯公司
Serotonin (5‐HT) has been shown to act as a morphogen in craniofacial and heart development and in the migration of neural crest derivatives. Some of these structures are capable of capturing 5‐HT during development, but nothing is known about the localization of the main monoamine degradation enzymes, monoamine oxidase (MAO) A and B, in these developing tissues. We generated a highly specific antibody to MAOB; immunoreactivity is entirely abolished in brain extracts or brain sections of mice lacking MAOB. From the use of this antibody and specific riboprobes, we report that MAOB is expressed early in a variety of neural crest derivatives, in facial sensory organs, and in the heart. From E11.5 to P0, MAOB was found to be strongly expressed in the following neural crest derivatives: the aorta, cranial mesenchyme (developing bones, sensory neurons of the cranial ganglia, cartilages, thyroid, and striate muscles), dental mesenchyme, several soft palate derivatives, and boundary cap cells (E11.5‐P4). Boundary cap cells contribute to the formation of nerve exit‐entry points between the central and the peripheral nervous systems. Several facial sensory organs also contained MAOB mRNA, protein, and activity. High MAOB expression was noted in the olfactory placode, the dorsal part of the olfactory epithelium, the olfactory nerve layer (probably the ensheathing glia), the cochlear ganglionic cells, the taste buds, and the Merkel cells in the vibrissae follicles. Finally, we found that MAOB is massively expressed in the pharyngeal organ, heart, liver, and mast cells. In contrast, MAOA expresssion was restricted to the sympathetic ganglia and to the meningeal and capillary blood vessels. The pattern of MAOB expression generally matched the previously reported patterns of expression of the plasma 5‐HT transporter expression or of the histamine biosynthetic enzyme L‐histidine decarboxylase, suggesting a role for MAOB in fine regulation of the levels of 5‐HT and histamine in the developing embryo. J. Comp. Neurol. 464:392–403, 2003. © 2003 Wiley‐Liss, Inc.