Application of Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Intrauterine Adhesions in Rats
Application of Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Intrauterine Adhesions in Rats
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DOI：
10.1159/000447857
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Zhang, Yujuan
中科院分区：
文献类型：
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作者：
Wang, Jianmei;Ju, Baohui;Zhang, Yujuan
目的：探讨骨髓间充质干细胞（BMSCs）移植治疗宫腔粘连（IUA）的疗效。方法：分离骨髓间充质干细胞，用绿色荧光蛋白标记。采用机械损伤法建立IUA模型。将48只大鼠随机分为对照组、IUA模型组、BMSCs静脉注射组和BMSCs宫内注射组，每组12只。第三代骨髓间充质干细胞通过尾静脉或宫内注射。分别于术后0 h、7 d、14 d、28 d处死3只大鼠，取双侧子宫进行实验。苏木精-伊红染色或Masson染色观察形态学变化。采用免疫组化法检测雌激素受体（ER）和孕激素受体（PR）。结果：BMSCs呈CD 44和CD 90特异性染色，而CD 45不表达。治疗前IUA模型组子宫内膜腺体数量明显减少，纤维化面积率明显增加（P
Aims: To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) transplantation on intrauterine adhesions (IUA). Methods: BMSCs were isolated and labeled by green fluorescence protein. IUA model was established by mechanical injury. 48 rats were randomly divided into control, IUA model, BMSCs vein injection and BMSCs intrauterine injection groups (n=12 in each group). The third generation of BMSCs was injected through tail vein or intrauterine. Three rats were killed at time 0 h, 7 d, 14 d and 28 d and bilateral uterus were obtained at each time points for the subseqent experiments. Morphological changes were determined by hematoxylin-eosin staining or Masson staining. Estrogen receptor (ER) and progesterone receptor (PR) were detected by immunohistochemistry. Results: BMSCs were specifically stained by CD44 and CD90, but not by CD45. Before treatment, the numbers of endometrial glands were significantly decreased, while fibrosis area rate was increased in IUA model group (P