Highly selective and sensitive FRET based ratiometric two-photon fluorescent probe for endogenous β-galactosidase detection in living cells and tissues
Highly selective and sensitive FRET based ratiometric two-photon fluorescent probe for endogenous β-galactosidase detection in living cells and tissues
复制标题
基于 FRET 的高选择性和灵敏的比率双光子荧光探针，用于活细胞和组织中内源性 β-半乳糖苷酶检测
DOI：
10.1016/j.microc.2020.105046
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发表时间：
2020
影响因子：
4.8
通讯作者：
Jinghua Yu
中科院分区：
文献类型：
--
作者：
Xianzhe Wei;Xiao-Xiao Hu;Li-Li Zhang;Jin Li;Jianrong Wang;Ping Wang;Zhiling Song;Jing Zhang;Mei Yan;Jinghua Yu
活体检测活体细胞和组织中异常的β-半乳糖苷酶(β-GAL)是早期诊断和治疗卵巢癌的迫切需要。基于FRET的比率双光子荧光探针FTR-βGalF具有发射位移大、响应快、细胞毒性低等特点，可灵敏地检测活体β-Gal活性。FTR-βGals是以一种萘衍生物为能量供体、一种Rhodol衍生物为能量受体设计的，为FRET提供了一种有效的FRET策略。值得注意的是，FTR-β半乳糖被成功地应用于β-GAL的TP成像，其比值信号在540-450∼nm之间迅速从0.15转换到5.9时( 40倍)。此外，FTR-βGals被用来显示当组织穿透达到30-150 μm时肿瘤中的Gal-Gal水平。因此，FTR-β-Gals适合于临床检测活细胞和组织，作为一种有前景的诊断各种相关癌症的策略。
In vivo measurement of abnormal β-galactosidase (β-gal) in living cells and tissues is urgently required for early diagnosis and treatment of primary ovarian cancer. Herein, a FRET-based ratiometric two-photon fluorescent probeFTR-βgalwith a large emission shift, rapid response and low cytotoxicity was developed for sensitively detecting β-gal activity in vivo.FTR-βgalwas designed with a naphthalene derivative as the energy donor and a rhodol derivative as the energy acceptor, which provided an efficient FRET strategy. Remarkably,FTR-βgalwas successfully applied in TP imaging of β-gal with the ratio signal rapidly transform from 0.15 to 5.9 (∼40-fold) between 540 nm and 450 nm. Furthermore,FTR-βgalwas used to visualize β-gal levels in tumor upon tissue penetration of 30–150 μm achieved. Therefore,FTR-βgalwas applicable for clinical detection in living cells and tissues as a promising strategy for diagnosis of various associated cancers.