Genetic developmental timing revealed by inter-species transplantations in fish
Genetic developmental timing revealed by inter-species transplantations in fish
复制标题
DOI：
10.1242/dev.192500
复制
发表时间：
2020-11-01
期刊：
影响因子：
4.6
通讯作者：
Centanin, Lazaro
中科院分区：
文献类型：
--
作者：
Franziska Fuhrmann, Jana;Buono, Lorena;Centanin, Lazaro
从受精卵到胚胎的过程涉及细胞类型、组织和器官的协调形成。发育模块包含由自给自足的遗传程序指定的离散单元，这些单元可以在胚胎发生过程中相互作用。在这里，我们利用两种远缘硬骨鱼斑马鱼 (Danio redo) 和青鳉 (Oryzias latipes) 之间胚胎发育的不同跨度（分别为 3 天和 9 天）来探索模块化原理。我们报告说，物种间囊胚移植导致由供体细胞形成的视网膜异位形成——一个模块。我们证明视网膜发育所需的时间遵循遗传程序：青鳉中的异位斑马鱼视网膜随着斑马鱼的动态而发育。异源移植导致供体视网膜和宿主生物体之间的时间脱钩，这可以通过需要视网膜-宿主相互作用的两种范例来说明：晶状体募集和视网膜顶盖投影。我们的结果揭示了一种新的实验系统，用于解决胚胎发育过程中的时间解耦问题，并强调了协调器官发生的很大程度上自主但相互关联的发育模块的存在。
The path from a fertilised egg to an embryo involves the coordinated formation of cell types, tissues and organs. Developmental modules comprise discrete units specified by self-sufficient genetic programs that can interact with each other during embryogenesis. Here, we have taken advantage of the different span of embryonic development between two distantly related teleosts, zebrafish (Danio redo) and medaka (Oryzias latipes) (3 and 9 days, respectively), to explore modularity principles. We report that inter-species blastula transplantations result in the ectopic formation of a retina formed by donor cells - a module. We show that the time taken for the retina to develop follows a genetic program: an ectopic zebrafish retina in medaka develops with zebrafish dynamics. Heterologous transplantation results in a temporal decoupling between the donor retina and host organism, illustrated by two paradigms that require retina-host interactions: lens recruitment and retino-tectal projections. Our results uncover a new experimental system for addressing temporal decoupling along embryonic development, and highlight the presence of largely autonomous but interconnected developmental modules that orchestrate organogenesis.