Ancestral expression patterns and evolutionary diversification of YABBY genes in angiosperms.
Ancestral expression patterns and evolutionary diversification of YABBY genes in angiosperms.
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DOI：
10.1111/j.1365-313x.2011.04570.x
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发表时间：
2011-07
期刊：
影响因子：
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通讯作者：
T. Yamada;S. Yokota;Y. Hirayama;R. Imaichi;Masahiro Kato;C. Gasser
中科院分区：
文献类型：
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作者：
T. Yamada;S. Yokota;Y. Hirayama;R. Imaichi;Masahiro Kato;C. Gasser
种子植物中的侧向器官生长部分地由转录因子的YABBY（YAB）和III类同源结构域/亮氨酸拉链（HD-ZIPIII）家族的成员控制。HD-ZIPIII基因似乎在这些器官中发挥保守的作用，但YAB基因已经多样化，该家族的一些成员在叶，心皮或胚珠珠被中具有专门的功能。祖先的表达模式和分歧的各类YAB基因的时间仍然有待建立。我们分离并评估了一个HD-ZIPIII和五个YAB基因的表达，这五个基因代表了来自Cabomba caroliniana的五个主要YAB基因类别，Cabomba caroliniana是最早发散的被子植物。与在真双子叶植物中的观察一致，C. caroliniana的表达在叶的远轴区，在那里出现新的薄片节，表达模式是相互排斥的HD-ZIPIII，表明这些表达模式是祖先。真双子叶植物和C. caroliniana，表明这些模式是原始的。然而，CRC基因在C. caroliniana中，在雄蕊中也有表达，而在另一个较早分化的种Amborella spanipoda中也有表达，表明心皮特异性表达是在睡莲目分化后获得的。YAB基因的表达数据和同源性表明，祖先YAB基因在侧器官的增殖组织中表达。
Lateral organ growth in seed plants is controlled in part by members of the YABBY (YAB) and class III homeodomain/leucine zipper (HD-ZIPIII) families of transcription factors. HD-ZIPIII genes appear to play a conserved role in such organs, but YAB genes have diversified, with some members of the family having specialized functions in leaves, carpels or ovule integuments. The ancestral expression patterns and timing of divergence of the various classes of YAB genes remain to be established. We isolated and evaluated the expression of one HD-ZIPIII and five YAB genes representing the five major YAB gene classes from Cabomba caroliniana, a member of the earliest-diverging angiosperms. Consistent with observations in eudicots, the FILAMENTOUS FLOWER (FIL) and YABBY5 (YAB5) genes of C. caroliniana were expressed in the abaxial regions of the leaf where new laminar segments arise, and the patterns of expression were mutually exclusive to those of HD-ZIPIII, indicating that these expression patterns are ancestral. Expression of CRABS CLAW (CRC) in the abaxial carpel wall, and of INNER NO OUTER (INO) in the abaxial outer integument of ovules was also conserved between eudicots and C. caroliniana, indicating that these patterns are primitive. However, the CRC gene was also expressed in other floral organs in C. caroliniana, and expression in stamens was also observed in another early-diverging species, Amborella trichopoda, indicating that carpel-specific expression was acquired after divergence of the Nymphaeales. The expression data and phylogeny for YAB genes suggest that the ancestral YAB gene was expressed in proliferating tissues of lateral organs.