Oncogenicity of virulent Marek's disease virus cloned as bacterial artificial chromosomes
Oncogenicity of virulent Marek's disease virus cloned as bacterial artificial chromosomes
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DOI：
10.1128/jvi.78.23.13376-13380.2004
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Nair, VK
中科院分区：
文献类型：
--
作者：
Petherbridge, L;Brown, AC;Nair, VK
马立克氏病病毒(MDV)是一种致癌的甲型疱疹病毒，可引起家禽T细胞淋巴瘤。本文报道了将迷你F载体序列插入到U(S)2基因座构建高致瘤性RB-IB菌株的细菌人工染色体克隆。由两个BAC克隆重组的MDV诱导了与野生型病毒类似的快速发病的淋巴瘤。由另一个BAC克隆重组而成的病毒，其内部和末端唯一的长重复序列有7.7 kBP的缺失，提示该缺失区域可能与致瘤性有关。MDV致癌BAC克隆的产生是解开该病毒致癌决定因素的重要一步。
Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-IB strain by inserting mini-F vector sequences into the U(S)2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.