DNA methylation errors in cloned mice disappear with advancement of aging.
DNA methylation errors in cloned mice disappear with advancement of aging.
复制标题
克隆小鼠的DNA甲基化错误随着年龄的增长而消失。
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
K. Shiota
中科院分区：
文献类型：
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作者：
S. Senda;T. Wakayama;Yoshikazu Arai;Y. Yamazaki;J. Ohgane;Satoshi Tanaka;N. Hattori;R. Yanagimachi;K. Shiota
克隆动物有各种各样的健康问题。异常的DNA甲基化是这些问题的可能原因。限制性标志基因组扫描（RLGS）使我们能够同时分析1,000多个CpG岛，证明所有克隆新生儿都有异常的DNA甲基化。为了研究这种畸变是否在克隆个体的整个生命中持续存在，我们以肾细胞为代表，检测了新生（19.5 dpc，n=2）、成年（8-11月龄，n=3）和老年（23-27月龄，n=4）克隆小鼠的全基因组DNA甲基化状态。在成年组和老年组中，使用每组的成年建立者克隆的卵丘细胞作为核供体重复克隆。两个新生克隆中有三个甲基化位点异常，这与以前所有克隆新生儿都存在DNA甲基化异常的报道一致。有趣的是，我们可以在中年的三个成年克隆中的两个中检测到一个异常甲基化的位点，而在四个衰老克隆中没有检测到，这表明DNA甲基化的错误随着动物衰老的进展而消失。
Cloned animals have various health problems. Aberrant DNA methylation is a possible cause of the problems. Restriction landmark genomic scanning (RLGS) that enabled us to analyze more than 1,000 CpG islands simultaneously demonstrated that all cloned newborns had aberrant DNA methylation. To study whether this aberration persists throughout the life of cloned individuals, we examined genome-wide DNA methylation status of newborn (19.5 dpc, n=2), adult (8-11 months old, n=3), and aged (23-27 months old, n=4) cloned mice using kidney cells as representatives. In the adult and aged groups, cloning was repeated using cumulus cells of the adult founder clone of each group as nucleus donor. Two newborn clones had three with aberrantly methylated loci, which is consistent with previous reports that all cloned newborns had DNA methylation aberrations. Interestingly, we could detect only one aberrantly methylated locus in two of the three adult clones in mid-age and none of four senescent clones, indicating that errors in DNA methylation disappear with advancement of animals' aging.
影响因子：
32.4
作者：
Lee, DU;Agarwal, S;Rao, A
通讯作者：
Rao, A
影响因子：
8.6
作者：
Richardson, B
通讯作者：
Richardson, B
影响因子：
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作者：
Lu, QJ;Kaplan, M;Richardson, B
通讯作者：
Richardson, B