The nature and action of granulocyte-macrophage colony stimulating factors.
The nature and action of granulocyte-macrophage colony stimulating factors.
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DOI：
10.1182/blood.v56.6.947.bloodjournal566947
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发表时间：
1980-12
期刊：
影响因子：
20.3
通讯作者：
A. Burgess;D. Metcalf
中科院分区：
文献类型：
--
作者：
A. Burgess;D. Metcalf
粒细胞-巨噬细胞集落刺激因子（GM-CSF）刺激粒细胞和巨噬细胞的体外增殖和分化。现在已知该调节剂在造血调节的其他水平上发挥作用。 GM-CSF的异质性不仅与来源组织和体外生产方法有关，还与具有不同生物学特异性的分子功能亚类有关。大多数成年小鼠器官产生 GM-CSF（摩尔重量 23,000），但在胎儿条件培养基 (CM) 中检测到巨噬细胞 (M)-CSF，并从 L 细胞 CM 中分离出来。鼠内毒素血清似乎含有 M-CSF、GM-CSF 和 G-CSF，最后一种与对白血病细胞有活性的分化因子共同分级。已在多种 CM 中检测到对人体细胞有活性的人 GM-CSF、G-CSF 和 EO-CSF，但尚未纯化。同样，有一些祖细胞亚类可以对特定形式的人类活性脑脊液做出反应。从小鼠肺CM中分离的GM-CSF刺激多能祖细胞、红细胞、嗜酸性粒细胞和巨核细胞系列中的祖细胞的初始增殖，以及GM系列中的成熟细胞。虽然 GM-CSF 也能够刺激骨髓白血病细胞的分化，但其他因素在这方面似乎更有效。在正确评估该分子的生理功能之前，需要有关 GM-CSF 产生的调节、其对特定靶细胞作用的调节剂以及其体内作用的信息。
Granulocyte-macrophage colony stimulating factor (GM-CSF) stimulates the in vitro proliferation and differentiation of granulocytic and macrophage cells. This regulator is now known to act at other levels of hemopoietic regulation. The heterogeneity of GM-CSFs is not only related to the tissue of origin and the in vitro production method, but also to functional subclasses of the molecule that have distinct biologic specificities. Most adult mouse organs produce GM-CSF (mol wt 23,000), but a macrophage (M)-CSF has been detected in fetal conditioned medium (CM) and isolated from L-cell CM. Murine endotoxin serum appears to contain a M-CSF, GM-CSF, and G-CSF, the last of which cofractionates with a differentiation factor active on leukemic cells. Human GM-CSFs, G-CSF, and EO-CSFs active on human cells have been detected in a variety of CM, but as yet none have been purified. Again, there are subclasses of progenitor cells that respond to particular forms of human active CSFs. GM-CSF isolated from mouse lung CM stimulates multipotential progenitor cells, the initial proliferatin of progenitors in the erythroid, eosinophil, and megakaryocyte series, as well as mature cells in the GM series. While GM-CSF is also able to stimulate the differentiation of myeloid leukemic cells, other factors appear to be more potent in this respect. Information on the regulation of GM-CSF production, on the modulators of its action on specific target cells, and on its role in vivo will be required before the physiologic function of this molecule can be properly assessed.