A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis
A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis
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DOI：
10.1136/gutjnl-2013-304833
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发表时间：
2014-08-01
期刊：
影响因子：
24.5
通讯作者：
Vermeire, Severine
中科院分区：
文献类型：
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作者：
Machiels, Kathleen;Joossens, Marie;Vermeire, Severine
目的细菌在炎症性肠病（IBD）肠道炎症的发生和持续中起重要作用。与克罗恩病（CD）不同，在溃疡性结肠炎（UC）中，微生态失调已经得到了更好的表征，只有小的队列进行了研究，并显示了相互矛盾的数据。因此，我们在一个大型队列中评估了CD中描述的微生物特征是否也存在于UC中，以及我们是否可以确定UC中的主要生态失调。为了评估功能的影响，生态失调，我们量化的细菌metabolites.Design的主要微生物群从127 UC患者和87年龄和性别匹配的控制进行了分析，使用变性梯度凝胶电泳（DGGE）分析。使用实时PCR定量验证差异。结果基于DGGE分析，先前在CD中描述的微生物特征在UC中不存在。实时荧光PCR分析显示，人罗斯拜瑞氏菌的丰度较低（p
Objective Bacteria play an important role in the onset and perpetuation of intestinal inflammation in inflammatory bowel disease (IBD). Unlike in Crohn's disease (CD), in which dysbiosis has been better characterised, in ulcerative colitis (UC), only small cohorts have been studied and showed conflicting data. Therefore, we evaluated in a large cohort if the microbial signature described in CD is also present in UC, and if we could characterise predominant dysbiosis in UC. To assess the functional impact of dysbiosis, we quantified the bacterial metabolites.Design The predominant microbiota from 127 UC patients and 87 age and sex-matched controls was analysed using denaturing gradient gel electrophoresis (DGGE) analysis. Differences were quantitatively validated using real-time PCR. Metabolites were quantified using gas chromatography-mass spectrometry.Results Based on DGGE analysis, the microbial signature previously described in CD was not present in UC. Real-time PCR analysis revealed a lower abundance of Roseburia hominis (p