Contribution of endotoxin in macrophage cytokine response to ambient particles in vitro
Contribution of endotoxin in macrophage cytokine response to ambient particles in vitro
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DOI：
10.1080/152873902760125741
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发表时间：
2002-09-13
影响因子：
2.6
通讯作者：
Chan, CC
中科院分区：
文献类型：
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作者：
Huang, SL;Cheng, WL;Chan, CC
周围的颗粒物可能会引起肺部炎症，并随之发病。颗粒诱导的促炎细胞因子的体外产生已被用作颗粒毒性的指标。为了确定与颗粒物毒性相关的颗粒物成分，使用Andersen二歧式撞击器收集台湾中部的环境细颗粒物（PM2.5）和粗颗粒物（PM2.5-10），并在无内毒素的水中进行提取。将小鼠单核-巨噬细胞系RAW 264.7细胞暴露于40 μ g/ml的颗粒提取物16小时，并通过酶联免疫吸附测定（ELISA）测量培养基中的肿瘤坏死因子-α（TNF-α）。细胞活力均大于82%。粗颗粒比细颗粒刺激更高的TNF-α产生，这与更大的颗粒内毒素含量有关。多粘菌素B抑制42%由粗颗粒引起的TNF-α产生和32%由细颗粒引起的TNF-α产生。在细颗粒中，TNF-α的产生与锌含量呈负相关，而粗颗粒中没有元素与TNF-α的产生相关。结果表明，内毒素和其他成分可能是体外巨噬细胞产生TNF-α的重要因素。
Ambient particles may cause pulmonary inflammation with ensuing morbidity. Particle-induced production of proinflammatory cytokines in vitro has been used as an indicator of particle toxicity. To identify particle components that were related to particle toxicity, Andersen dichotomous impactors were used to collect ambient fine (PM2.5) and coarse (PM2.5-10) particles in central Taiwan with extraction in endotoxin-free water. Mouse monocyte-macrophage cell line RAW 264.7 cells were exposed to particle extracts at 40 mug/ml for 16 h, and tumor necrosis factor-alpha (TNF-alpha) was measured in the medium by enzyme-linked immunosorbent assay (ELISA). Cell viabilities were all greater than 82%. Coarse particles stimulated higher TNF-alpha production than fine particles, and this was associated with greater particulate endotoxin content. Polymyxin B inhibited 42% of TNF-alpha production elicited by coarse particles and 32 % of TNF-alpha production elicited by fine particles. In fine particles, TNF-alpha production was negatively correlated with Zn content, while no element in coarse particles correlated with TNF-alpha production. Results suggest that endotoxin and other components may be important factors for TNF-alpha production by macrophages in vitro.