phospholipase C gamma-1 is require downstream of vascular endothelial growth factor during arterial development
phospholipase C gamma-1 is require downstream of vascular endothelial growth factor during arterial development
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DOI：
10.1101/gad.1072203
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发表时间：
2003-06-01
影响因子：
10.5
通讯作者：
Weinstein, BM
中科院分区：
文献类型：
--
作者：
Lawson, ND;Mugford, JW;Weinstein, BM
在这项研究中，我们利用带有荧光标记血管的转基因斑马鱼来识别和表征突变体 (y10)，该突变体在动脉形成中表现出特定缺陷，但在静脉形成中没有。我们发现 y10 编码磷脂酶 C gamma-1 (plcg1)，这是一种已知的受体酪氨酸激酶信号传导效应子。我们进一步表明 plcg1(y10) 突变体胚胎无法对外源 Vegf 做出反应。我们的结果表明，在胚胎发育过程中，Plcg1 在 Vegf 受体下游特异性发挥作用，以控制动脉系统的形成。
In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find that y10 encodes phospholipase C gamma-1 (plcg1), a known effector of receptor tyrosine kinase signaling. We further show that plcg1(y10) mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.