The influenza virus protein PB1-F2 interacts with IKKβ and modulates NF-κB signalling.
The influenza virus protein PB1-F2 interacts with IKKβ and modulates NF-κB signalling.
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DOI：
10.1371/journal.pone.0063852
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
McCauley JW
中科院分区：
文献类型：
--
作者：
Reis AL;McCauley JW
PB1-F2, a protein encoded by a second open reading frame of the influenza virus RNA segment 2, has emerged as a modulator of lung inflammatory responses but the molecular mechanisms underlying this are only poorly understood. Here we show that PB1-F2 inhibits the activation of NF-κB dependent signalling pathways in luciferase reporter assays. PB1-F2 proteins from four different viruses interact with IKKβ in yeast two-hybrid assays and by co-immunoprecipitation. PB1-F2 expression did not inhibit IKKβ kinase activity or NF-κB translocation into the nucleus, but NF-κB binding to DNA was severely impaired in PB1-F2 transfected cells as assessed by Electrophoretic Mobility Shift Assay. Neither the N-terminal 57 amino acid truncated forms nor the C-terminus of PB1-F2 were able to inhibit NF-κB dependent signalling, indicating that the full length protein is necessary for the inhibition.
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