Production and diffusion of chloroplastic H2O2 and its implication to signalling
Production and diffusion of chloroplastic H2O2 and its implication to signalling
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DOI：
10.1093/jxb/erq171
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发表时间：
2010-08-01
影响因子：
6.9
通讯作者：
Krieger-Liszkay, Anja
中科院分区：
文献类型：
--
作者：
Mubarakshina, Maria M.;Ivanov, Boris N.;Krieger-Liszkay, Anja
过氧化氢（H2O2）被认为是一种重要的信号分子。这一功能有两个重要方面：H2O2的产生及其向作用部位的扩散。利用自旋捕获电子顺磁共振光谱和H2O2敏感的荧光染料研究了光合电子传递产生H2O2及其通过叶绿体被膜扩散的能力。有人发现，即使在低光强度下，叶绿体内产生的H2O2的一部分可以离开叶绿体，从而逃避位于叶绿体内的有效的抗氧化系统。叶绿体产生的H_2O_2和叶绿体外H_2O_2的出现随着光照强度和光照时间的增加而增加。可以检测到的叶绿体外的H2O2的量已被证明是高达5%的总H2O2内产生的叶绿体在高光强度。叶绿体产生的H2O2可以在这些细胞器外被检测到，这一事实对于理解叶绿体H2O2如何作为信号分子是一个重要的发现。
Hydrogen peroxide (H2O2) is recognized as an important signalling molecule. There are two important aspects to this function: H2O2 production and its diffusion to its sites of action. The production of H2O2 by photosynthetic electron transport and its ability to diffuse through the chloroplast envelope membranes has been investigated using spin trapping electron paramagnetic resonance spectroscopy and H2O2-sensitive fluorescence dyes. It was found that, even at low light intensity, a portion of H2O2 produced inside the chloroplasts can leave the chloroplasts thus escaping the effective antioxidant systems located inside the chloroplast. The production of H2O2 by chloroplasts and the appearance of H2O2 outside chloroplasts increased with increasing light intensity and time of illumination. The amount of H2O2 that can be detected outside the chloroplasts has been shown to be up to 5% of the total H2O2 produced inside the chloroplasts at high light intensities. The fact that H2O2 produced by chloroplasts can be detected outside these organelles is an important finding in terms of understanding how chloroplastic H2O2 can serve as a signal molecule.