Separation of six lipid classes on one thin-layer chromatogram.
Separation of six lipid classes on one thin-layer chromatogram.
复制标题
在一张薄层色谱上分离六种脂质。
DOI：
10.1016/s0021-9673(00)92542-1
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
S. C. Smith
中科院分区：
文献类型：
--
作者：
J. Hojnacki;S. C. Smith
样品在默克色谱板上，20×20厘米，硅胶被层析，直到溶剂，乙醚，冰醋酸和石油醚，b.p。38.5°~40.0°(100：3：97)，向下移位9 cm。干燥后，将平板放入乙醚和石油醚(3：97)中，使正面移动15厘米。然后将盘子风干，并喷洒罗丹明6G。分离出磷脂、胆固醇、游离脂肪酸、甘油三酯、胆固醇酯和角鲨烯。
The sample on a Merck chromatoplate, 20 X 20 cm, of silica gel was chromatographed until the solvent, ether, acetic acid and petroleum ether, b.p. 38.5 deg to 40.0 deg (100:3:97), had migrated 9 cm from the bottom. After drying the plate was placed in ether and petroleum ether (3:97) which allowed the front to migrate 15 cm. The plate was then air-dried and sprayed with Rhodamine 6G. Phospholipid, cholesterol, free fatty acids, triglyceride, cholesteryl ester and squalene were separated.