Dual-channel imaging system for singlet oxygen and photosensitizer for PDT.
Dual-channel imaging system for singlet oxygen and photosensitizer for PDT.
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DOI：
10.1364/boe.2.001233
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发表时间：
2011-04-15
影响因子：
3.4
通讯作者：
Davis SJ
中科院分区：
文献类型：
--
作者：
Lee S;Isabelle ME;Gabally-Kinney KL;Pogue BW;Davis SJ
开发了一种双通道光学系统，可以对光动力过程产生的单线态分子氧 (1O2) 磷光和光敏剂 (PS) 荧光进行空间分辨同步成像。当前的成像系统使用光谱辨别方法来区分峰值在 1.27 μm 附近的微弱 1O2 磷光和同样出现在该光谱区域的 PS 荧光。 1O2 发射的检测限是在组织样体模中浓度为 500 nM 苯并卟啉衍生物一酸 (BPD) 时测定的，观察到的这些信号与 PS 荧光成正比。荷瘤小鼠的初步体内图像表明，可以获得 1O2 和 PS 组织分布的同步图像。
A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen (1O2) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to differentiate the weak 1O2 phosphorescence that peaks near 1.27 μm from PS fluorescence that also occurs in this spectral region. The detection limit of 1O2 emission was determined at a concentration of 500 nM benzoporphyrin derivative monoacid (BPD) in tissue-like phantoms, and these signals observed were proportional to the PS fluorescence. Preliminary in vivo images with tumor laden mice indicate that it is possible to obtain simultaneous images of 1O2 and PS tissue distribution.