Epidermal growth factor reduces HER‐2 protein level in human ovarian carcinoma cells
Epidermal growth factor reduces HER‐2 protein level in human ovarian carcinoma cells
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表皮生长因子降低人卵巢癌细胞中 HER-2 蛋白水平
DOI：
10.1002/ijc.2910520226
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发表时间：
1992
影响因子：
6.4
通讯作者：
G. Daxenbichler
中科院分区：
文献类型：
--
作者：
C. Marth;T. Lang;M. Cronauer;W. Doppler;A. Zeimet;F. Bachmair;A. Ullrich;G. Daxenbichler
原癌基因HER-2（c-erbB-2/neu）在卵巢癌、子宫内膜癌和乳腺癌中的过度表达是预后不良的指标。表皮生长因子（EGF）受体和HER-2蛋白之间的相互作用已被描述。本研究的目的是阐明EGF对HER-2表达的影响。在人卵巢癌细胞系HTB-77、OVCAR-3、2780、SKOV-6、SKOV-8和2774以及人乳腺肿瘤细胞系SKBR-3中，通过ELISA测定总细胞p185 HER-2，而通过活细胞RIA测定表面p185 HER-2。用EGF（0.1-30 nM）或TGF-α（0.1-30 nM）刺激这些细胞系导致p185 HER-2表达显著降低。这种效应在HER-2表达正常的细胞中更为明显。在OVCAR-3细胞中观察到EGF降低p185 HER-2的mRNA水平，但在过表达细胞系HTB-77和SKBR-3中未观察到。有趣的是，EGF诱导的效应并不总是与生长刺激相关，并且与放射性配体测定检测到的EGF结合位点的数量无关。我们的数据表明，EGF治疗导致p185 HER-2下调。© 1992 Wiley利斯公司
Over‐expression of the proto‐oncogene HER‐2 (c‐erbB‐2/neu) in ovarian, endometrial, and mammary carcinoma is an indicator of poor prognosis. Interactions between the epidermal growth factor (EGF) receptor and the HER‐2 protein have been described. The aim of this study was to elucidate the effects of EGF on HER‐2 expression. In the human ovarian carcinoma cell lines HTB‐77, OVCAR‐3, 2780, SKOV‐6, SKOV‐8 and 2774, and the human mammary tumor cell line SKBR‐3, total cellular p185HER‐2 was determined by an ELISA, whereas the surface p185HER‐2 was measured with a living‐cell RIA. Stimulation of these cell lines with either EGF (0.1–30 nM) or TGF‐α (0.1–30 nM) led to a significant reduction in p185HER‐2 expression. The effect was more pronounced in cells with normal HER‐2 expression. A reduction of mRNA levels for p185HER‐2 by EGF was observed in OVCAR‐3 cells but not in the over‐expressing lines HTB‐77 and SKBR‐3. Interestingly, the EGF‐induced effect was not always associated with growth stimulation and was not correlated with the number of EGF binding sites detected by a radioligand assay. Our data indicate that EGF treatment results in a down‐regulation of p185HER‐2. © 1992 Wiley‐Liss, Inc.
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影响因子：
11.2
作者：
A. Berchuck;R. Whitaker;G. Olt;J. Soper;D. Clarke‐Pearson;A. Kamel;A. Kamel;R. Bast;B. Kerns;R. Kinney;R. Dodge;P. Marks;S. McKenzie;S. Yin
通讯作者：
A. Berchuck;R. Whitaker;G. Olt;J. Soper;D. Clarke‐Pearson;A. Kamel;A. Kamel;R. Bast;B. Kerns;R. Kinney;R. Dodge;P. Marks;S. McKenzie;S. Yin
影响因子：
56.9
作者：
SLAMON, DJ;CLARK, GM;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
假设激素的实验方法：检测 neu 原癌基因的候选配体。
DOI：
10.1073/pnas.86.9.3179
发表时间：
1989
影响因子：
11.1
作者：
Yarden,Y;Weinberg,RA
通讯作者：
Weinberg,RA
影响因子：
2.9
作者：
GOLDMAN, R;BENLEVY, R;YARDEN, Y
通讯作者：
YARDEN, Y
影响因子：
56.9
作者：
SLAMON, DJ;GODOLPHIN, W;PRESS, MF
通讯作者：
PRESS, MF