Evidence of a novel human coronavirus that is associated with respiratory tract disease in infants and young children
Evidence of a novel human coronavirus that is associated with respiratory tract disease in infants and young children
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DOI：
10.1086/428138
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发表时间：
2005-02-15
影响因子：
6.4
通讯作者：
Kahn, JS
中科院分区：
文献类型：
--
作者：
Esper, F;Weibel, C;Kahn, JS
背景导致相当大比例呼吸道疾病的病原体尚未确定。我们试图确定新型人类冠状病毒（HCoV）是否在康涅狄格州纽黑文市流行，如果是，它们是否与婴幼儿呼吸道疾病相关。我们开发了一种基于聚合酶链反应（PCR）的方法，用于筛查有症状儿童呼吸道标本。设计了靶向在遗传多样性动物CoV和HCoV中保守的复制酶1a基因区域的PCR探针。使用这些探针，我们确定了一种新的HCoV的基因组序列，命名为“纽黑文冠状病毒”（HCoV-NH）。此后，我们设计了特异性探针，用于筛查儿童呼吸道标本
Background. The etiological agents responsible for a substantial proportion of respiratory tract diseases have not been identified. We sought to determine whether novel human coronaviruses (HCoVs) are circulating in New Haven, Connecticut, and, if so, whether they are associated with respiratory tract disease in infants and young children.Methods. We developed a polymerase chain reaction (PCR)-based approach for screening specimens from the respiratory tracts of symptomatic children. PCR probes that target regions of the replicase 1a gene that are conserved among genetically diverse animal CoVs and HCoVs were designed. Using these probes, we identified genomic sequences of a novel HCoV, designated "New Haven coronavirus" (HCoV-NH). Thereafter, we designed specific probes to screen respiratory specimens from children