Systematic functional comparative analysis of four single-stranded DNA-binding proteins and their affection on viral RNA metabolism.
Systematic functional comparative analysis of four single-stranded DNA-binding proteins and their affection on viral RNA metabolism.
复制标题
四种单链 DNA 结合蛋白的系统功能比较分析及其对病毒 RNA 代谢的影响
DOI：
10.1371/journal.pone.0055076
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Shi H;Zhang Y;Zhang G;Guo J;Zhang X;Song H;Lv J;Gao J;Wang Y;Chen L;Wang Y
The accumulation of single-stranded DNA-binding (SSB) proteins is essential for organisms and has various applications. However, no study has simultaneously and systematically compared the characteristics of SSB proteins. In addition, SSB proteins may bind RNA and play an unknown biological role in RNA metabolism. Here, we expressed a novel species of SSB protein derived from Thermococcus kodakarensis KOD1 (KOD), as well as SSB proteins from Thermus thermophilus (TTH), Escherichia coli, and Sulfolobus Solfataricus P2 (SSOB), abbreviated kod, tth, bl21, and ssob, respectively. These SSB proteins could bind ssDNA and viral RNA. bl21 resisted heat treatment for more than 9 h, Ssob and kod could withstand 95°C for 10 h and retain its ssDNA- and RNA-binding ability. Four SSB proteins promoted the specificity of the DNA polymerase in PCR-based 5- and 9-kb genome fragment amplification. kod also increased the amplification of a 13-kb PCR product, and SSB protein–bound RNA resisted Benzonase digestion. The SSB proteins could also enter the host cell bound to RNA, which resulted in modulation of viral RNA metabolism, particularly ssob and bl21.
登录
查看更多内容
DOI：
10.1073/pnas.1133325100
发表时间：
2003-08-05
影响因子：
11.1
作者：
Davydova, EK;Rothman-Denes, LB
通讯作者：
Rothman-Denes, LB
影响因子：
14.9
作者：
Perales, C;Cava, F;Berenguer, J
通讯作者：
Berenguer, J
影响因子：
2.2
作者：
Simpson, JM;McCracken, VJ;Mackie, RI
通讯作者：
Mackie, RI
影响因子：
14.9
作者：
Fedorov, Roman;Witte, Gregor;Urbanke, Claus;Manstein, Dietmar J.;Curth, Ute
通讯作者：
Curth, Ute
影响因子：
4.4
作者：
Takagi, M;Nishioka, M;Imanaka, T
通讯作者：
Imanaka, T