Cloning of the flap endonuclease-1 gene in Bombyx mori and identification of an antiapoptotic function.
Cloning of the flap endonuclease-1 gene in Bombyx mori and identification of an antiapoptotic function.
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DOI：
10.1089/dna.2011.1224
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发表时间：
2011-09
影响因子：
3.1
通讯作者：
M. Pan;Juan Du;Jin-Ye Zhang;Mao-Hua Huang;Tingxiu Li;H. Cui;Cheng Lu
中科院分区：
文献类型：
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作者：
M. Pan;Juan Du;Jin-Ye Zhang;Mao-Hua Huang;Tingxiu Li;H. Cui;Cheng Lu
皮瓣内切酶-1 （flap endonuclease-1, FEN-1）基因参与DNA的复制和修复，在正常生长条件下维持基因组的稳定性和DNA复制的准确性。然而，FEN-1在细胞凋亡和癌症发展中也起着重要作用。从家蚕中克隆到BmFEN-1基因，全长1343 bp， ORF为1143 bp（123-1266）。它由7个内含子和8个外显子组成，编码一个含有380个氨基酸的蛋白质，具有典型的XPG结构域。n端基序位于氨基酸95-105，增殖细胞核抗原相互作用基序位于氨基酸337-344。RNA干扰介导的BmFEN-1表达减少诱导BmE-SWU1细胞S期细胞周期阻滞。提示BmFEN-1具有抑制细胞凋亡、促进细胞增殖的作用。
The flap endonuclease-1 (FEN-1) gene is involved in DNA replication and repair, and it maintains genomic stability as well as the accuracy of DNA replication under normal growth conditions. However, FEN-1 also plays an important role in apoptosis and cancer development. We cloned the BmFEN-1 gene from Bombyx mori, which was 1343 bp in length and possessed an 1143 bp ORF (123-1266). It consists of seven introns and eight exons that encode a protein with 380 amino acids that has the typical XPG domain. The N-terminal motif is located at amino acids 95-105, and the proliferating cell nuclear antigen interaction motif is located at amino acids 337-344. RNA interference-mediated reduction of BmFEN-1 expression induced cell cycle arrest in S phase in BmE-SWU1 cells. These results suggest that BmFEN-1 can inhibit apoptosis and promote cell proliferation.