Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection
Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection
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DOI：
10.1016/j.jviromet.2012.09.012
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Kroon, Erna Geessien
中科院分区：
文献类型：
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作者：
de Oliveira Rocha, Eliseu Soares;de Oliveira, Jaquelline Germano;Kroon, Erna Geessien
登革热病毒（DENV）是世界上最流行的虫媒病毒，主要发生在热带地区。由于临床表现通常为非特异性发热性疾病，因此实验室诊断对于确认DENV感染和进行流行病学研究至关重要。采用四种DENV血清型的重组包膜(E)抗原建立免疫球蛋白G酶联免疫吸附试验（IgG-ELISA）。为了评估IgG-ELISA，研究了一组先前通过斑块减少中和试验（PRNT）测试的血清样本，以检测针对四种DENV血清型的抗e抗体的存在。发现IgG-ELISA在受试者工作特征（ROC）优化截止点上具有灵敏度（91%）和特异性（98%），表现出高性能和良好的指标。两种检测方法之间的一致性达到97%，只有21/704（3%）样品不一致。本研究结果表明，中和抗体滴度与IgG-ELISA值之间存在适度的相关性。上述结果提示，基于重组蛋白的IgG-ELISA是一种适合登革热病毒感染常规血清诊断、监测和血清流行病学研究的方法。(C) 2012 Elsevier B.V.版权所有
Dengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704(3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections. (C) 2012 Elsevier B.V. All rights reserved.