QTL detection for node of first fruiting branch and its height in upland cotton (Gossypium hirsutum L.)
QTL detection for node of first fruiting branch and its height in upland cotton (Gossypium hirsutum L.)
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DOI：
10.1007/s10681-012-0720-2
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发表时间：
2012-12-01
期刊：
影响因子：
1.9
通讯作者：
Wang, Qinglian
中科院分区：
文献类型：
--
作者：
Li, Chengqi;Wang, Changbiao;Wang, Qinglian
早熟棉的选育和推广，是优化农业结构、解决占用耕地、利用粮食和棉花的矛盾的关键。第一果枝节数(NFFB)和果枝高度(HNFFB)可作为衡量棉花早熟性的重要指标。本研究以早熟陆地棉品种白棉2号为试验材料。分别以晚熟材料(TM-1)和中熟品种(CIR12)为母本进行杂交。用复合区间作图法(CIM)和混合模型作图法(MCIM)对白棉2×TM-1和白棉2×CIR12两个F-2：3群体进行了NFFB和HNFFB的QTL定位。在两个群体中检测到8个NFFB QTL(3个提示和5个显著)和6个HNFFB QTL(3个提示和3个显著)，分别位于C1(A1)、C5(A5)、C6(A6)、C9(A9)、C11(A11)、C13(A13)、C17(D3)、C25(D6)、C12(A12)/C26(D12)和LG1上。QTL分析结果与分离分析结果有一定的一致性。CIM和MCIM同时检测到NFFB的qNFFB-17-I和HNFFB的qHNFFB-17-I两个主效QTL。具有较高的可靠性，可用于棉花早熟性的分子标记辅助育种。单标记分析表明，CGR5222等11个分子标记与NFFB和/或HNFFB连锁。
The selection and popularization of early-maturing cotton is paramount in resolving the conflict between occupying farmlands utilizing grain and cotton while optimizing agricultural structure. Node of first fruiting branch (NFFB) and its height (HNFFB) can be used as the important indicators to measure cotton earliness. In this study, Baimian2, an early-maturing upland cotton (Gossypium hirsutum L.) variety, was used as the female parent crossed with a late-maturing material (TM-1) and intermediate-maturing variety (CIR12), respectively. Quantitative trait locus (QTL) detection for NFFB and HNFFB was performed within the two F-2:3 populations, Baimian2 x TM-1 and Baimian2 x CIR12 by composite interval mapping (CIM) and mixed model CIM (MCIM). Eight QTL (three suggestive and five significant) for NFFB and six QTL (three suggestive and three significant) for HNFFB were detected in both populations, located on C1(A1), C5(A5), C6(A6), C9(A9), C11(A11), C13(A13), C17(D3), C25(D6), C12(A12)/C26(D12) and LG1. Results of QTL are consistent with separation analysis to some extent. The two significant major QTL, qNFFB-17-I for NFFB and qHNFFB-17-I for HNFFB, were detected simultaneously by CIM and MCIM. They had high reliability and could be used for the marker-assisted breeding to improve cotton earliness. Single marker-analysis showed that 11 molecular markers, such as CGR5222, are linked with NFFB and/or HNFFB.