Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis.
Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis.
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DOI：
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发表时间：
1991-04
期刊：
影响因子：
4.6
通讯作者：
J. Christian;J. McMahon;A. McMahon;R. Moon
中科院分区：
文献类型：
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作者：
J. Christian;J. McMahon;A. McMahon;R. Moon
在两栖类胚胎中，基本体型的形成取决于中胚层的位置差异。虽然参与中胚层诱导的多肽生长因子已经初步确定，但需要额外的信号才能在该组织中产生模式。我们已经分离到一个与Wnt-1相关的基因，命名为Xwnt-8，它在中胚层诱导的生长因子作用下被激活。XWNT-8转录本是瞬时表达的，在原肠形成期间表达最丰富，此时表达主要限于腹侧中胚层细胞。经锂暴露的背侧胚胎显示Xwnt-8mRNA水平显著降低，支持Xwnt-8的表达与腹侧中胚层细胞命运之间的相关性。令人惊讶的是，Xwnt-8在胚胎中的异位表达导致了背部前部增强的表型。这些发现表明，XWNT-8可能是一种次级信号转导因子，它是对中胚层诱导因子的反应，并参与了中胚层图案化的早期步骤。
In amphibian embryos, formation of the basic body plan depends on positional differences in the mesoderm. Although peptide growth factors involved in mesoderm induction have tentatively been identified, additional signals are required to generate pattern in this tissue. We have isolated a Xenopus cDNA for a Wnt-1 related gene, designated Xwnt-8, which is activated in response to mesoderm-inducing growth factors. Xwnt-8 transcripts are transiently expressed, being most abundant during gastrulation at which time expression is confined primarily to ventral mesodermal cells. Embryos dorsoanteriorized by exposure to lithium exhibit greatly reduced levels of Xwnt-8 mRNA, supporting a correlation between Xwnt-8 expression and a ventral mesodermal cell fate. Surprisingly, ectopic expression of Xwnt-8 in embryos causes a dorsoanterior-enhanced phenotype. These findings suggest that Xwnt-8 may be a secondary signalling agent which is produced in response to mesoderm-inducing factors and is involved in the early steps of mesodermal patterning.